Detección del virus SARS-CoV-2 a través de la prueba de amplificación isotérmica mediada por bucle con transcripción reversa (RT-LAMP)
Abstract The purpose of the present study was to standardize a fast and effortless methodology for the detection of SARS-CoV-2, by reverse transcription loop-mediated isothermal nucleic acid amplification technique (RT-LAMP) for COVID-19 diagnosis. Standardization was made in RNA samples previously...
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| Autores principales: | , , , , |
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| Formato: | Digital |
| Lenguaje: | spa |
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UNIVERSIDAD ANTONIO NARIÑO
2024
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| Materias: | |
| Acceso en línea: | https://revistas.uan.edu.co/index.php/saywa/article/view/1506 |
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| Sumario: | Abstract
The purpose of the present study was to standardize a fast and effortless methodology for the detection of SARS-CoV-2, by reverse transcription loop-mediated isothermal nucleic acid amplification technique (RT-LAMP) for COVID-19 diagnosis. Standardization was made in RNA samples previously confirmed by RT-qPCR using the Charité/Berlin protocol. We detail the steps of the technique and highlight use of S-123 primers. In conclusion, RT-LAMP with S-123 primers for SARS-CoV-2 in RNA samples, shows a high level of performance compared with gold standard technique RT-qPCR.
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