Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica
Salmonella Typhimurium Monophasic variant (STVM), has become a problem in public health globally due to its sudden rise of dominant clones and fast spread. In Colombia, the National Institute of Health reported the circulation of this variant since 2015, ranked sixth among the 20 Salmonella serovars...
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| Formato: | Tesis y disertaciones (Maestría y/o Doctorado) |
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Universidad Antonio Nariño
2022
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| Acceso en línea: | http://repositorio.uan.edu.co/handle/123456789/6383 |
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| _version_ | 1812648132852318208 |
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| author | Cuenca Arias, Paloma |
| author2 | Wiesner Reyes, Magdalena |
| author_facet | Wiesner Reyes, Magdalena Cuenca Arias, Paloma |
| author_sort | Cuenca Arias, Paloma |
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| description | Salmonella Typhimurium Monophasic variant (STVM), has become a problem in public health globally due to its sudden rise of dominant clones and fast spread. In Colombia, the National Institute of Health reported the circulation of this variant since 2015, ranked sixth among the 20 Salmonella serovars isolated with the highest frequency. Our aim was to characterize genotypically the STVM Colombian isolates confirming the clone-type spreading nationwide. In this study, we included a whole-genome sequencing dataset of 21 Colombian clinical isolates of STVM that were analyzed by using bioinformatics and comparative genomics tools. Our results suggest high genomic plasticity in STVM strains mediated by the acquisition of mobile and conjugative genetic elements, conferring heavy metal tolerance and antibiotic resistance. Moreover, the absence of genes involved in adhesion, invasion, and colonization processes suggest an adaptation process of the new serovar, independently of clonal lineage, source, or geographical origin. |
| format | Tesis y disertaciones (Maestría y/o Doctorado) |
| id | repositorio.uan.edu.co-123456789-6383 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-63832024-10-09T23:12:55Z Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica Cuenca Arias, Paloma Wiesner Reyes, Magdalena Arenas Suárez, Nelson Enrique Salmonella Typhimurium Variante monofásica Profago Antibióticoresistencia Plásmidos Plasticidad genómica Mutaciones Flagelo Secuenciotipo SPI SGI 540 Salmonella Typhimurium Monophasic variant Prophage Antibioticresistance Plasmids Genomic plasticity Mutations Flagellum Sequence SPI SGI Salmonella Typhimurium Monophasic variant (STVM), has become a problem in public health globally due to its sudden rise of dominant clones and fast spread. In Colombia, the National Institute of Health reported the circulation of this variant since 2015, ranked sixth among the 20 Salmonella serovars isolated with the highest frequency. Our aim was to characterize genotypically the STVM Colombian isolates confirming the clone-type spreading nationwide. In this study, we included a whole-genome sequencing dataset of 21 Colombian clinical isolates of STVM that were analyzed by using bioinformatics and comparative genomics tools. Our results suggest high genomic plasticity in STVM strains mediated by the acquisition of mobile and conjugative genetic elements, conferring heavy metal tolerance and antibiotic resistance. Moreover, the absence of genes involved in adhesion, invasion, and colonization processes suggest an adaptation process of the new serovar, independently of clonal lineage, source, or geographical origin. Salmonella Typhimurium variante Monofásica (STVM) se ha convertido en un problema de salud pública a nivel global, por la sucesiva aparición de clones dominantes y rápida diseminación. En Colombia, el Instituto Nacional de Salud, reporta la circulación de esta variante para el año 2015, posicionada en sexto lugar entre los 20 serovares aislados con mayor frecuencia de Salmonella spp. El objetivo del presente trabajo fue caracterizar genotípicamente los aislamientos de STVM colombianos confirmando el tipo de clon circulante a nivel nacional. En el presente estudio se incluyeron los genomas completos de 21 aislamientos clínicos colombianos de STVM, que se analizaron mediante herramientas computacionales y de genómica comparativa. Los resultados sugieren una alta plasticidad genómica en cepas de STVM mediada por la adquisición de elementos genéticos móviles y conjugativos que confieren tolerancia a metales pesados y resistencia a antibióticos. También, se observó la ausencia de genes involucrados en procesos de adhesión, invasión y colonización, reflejando procesos de adaptación de la nueva serovariedad independiente del linaje clonal, fuente u origen geográfico. Magíster en Bioquímica Maestría Presencial Investigación 2022-04-30T16:01:44Z 2022-04-30T16:01:44Z 2021-11-24 Tesis y disertaciones (Maestría y/o Doctorado) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_bdcc http://purl.org/coar/version/c_970fb48d4fbd8a85 Tesis/Trabajo de grado - Monografía - Maestría http://repositorio.uan.edu.co/handle/123456789/6383 Achard, M. E. S., Stafford, S. L., Bokil, N. J., Chartres, J., Bernhardt, P. V., Schembri, M. A., … McEwan, A. G. (2012). Copper redistribution in murine macrophages in response to Salmonella infection. The Biochemical Journal, 444(1), 51–57. doi:10.1042/BJ20112180 Albanna, A., Sim, M., Hoskisson, P. A., Gillespie, C., Rao, C. V., & Aldridge, P. D. (2018). Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences. Scientific Reports, 8(1), 16705. doi:10.1038/s41598-018-35005-2 Arenas, N. E., & Moreno Melo, V. (2018). Producción pecuaria y emergencia de antibiótico resistencia en Colombia: Revisión sistemática. Infectio, 22(2), 110. doi:10.22354/in.v22i2.717 Arnott, A., Wang, Q., Bachmann, N., Sadsad, R., Biswas, C., Sotomayor, C., … Sintchenko, V. (2018). Multidrug-Resistant Salmonella enterica 4,[5],12:i:- Sequence Type 34, New South Wales, Australia, 2016-2017. Emerging Infectious Diseases, 24(4), 751–753. doi:10.3201/eid2404.171619 Ashton, P. M., Owen, S. V., Kaindama, L., Rowe, W. P. M., Lane, C. R., Larkin, L., … Dallman, T. J. (2017). Public health surveillance in the UK revolutionises our understanding of the invasive Salmonella Typhimurium epidemic in Africa. Genome Medicine, 9(1), 92. doi:10.1186/s13073-017-0480-7 Barco, L., Longo, A., Lettini, A. A., Cortini, E., Saccardin, C., Minorello, C., … Ricci, A. (2014). Molecular characterization of “inconsistent” variants of Salmonella Typhimurium isolated in Italy. Foodborne Pathogens and Disease, 11(6), 497–499. doi:10.1089/fpd.2013.1714 Barreto, M., Castillo-Ruiz, M., & Retamal, P. (2016). [Salmonella enterica: a review or the trilogy agent, host and environment and its importance in Chile]. Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia, 33(5), 547– 557. doi:10.4067/S0716-10182016000500010 Bäumler, A. J., Tsolis, R. M., & Heffron, F. (1996). Contribution of fimbrial operons to attachment to and invasion of epithelial cell lines by Salmonella typhimurium. Infection and Immunity, 64(5), 1862–1865. doi:10.1128/iai.64.5.1862-1865.1996 Bearson, B. L., Trachsel, J. M., Shippy, D. C., Sivasankaran, S. K., Kerr, B. J., Loving, C. L., … Bearson, S. M. D. (2020). The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1. Genes, 11(11). doi:10.3390/genes11111291 Boland, C., Bertrand, S., Mattheus, W., Dierick, K., Jasson, V., Rosseel, T., … Wattiau, P. (2015). Extensive genetic variability linked to IS26 insertions in the fljB promoter region of atypical monophasic variants of Salmonella enterica serovar Typhimurium. Applied and Environmental Microbiology, 81(9), 3169–3175. doi:10.1128/AEM.00270-15 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Maestría en Bioquímica Facultad de Ciencias Bogotá - Circunvalar |
| spellingShingle | Salmonella Typhimurium Variante monofásica Profago Antibióticoresistencia Plásmidos Plasticidad genómica Mutaciones Flagelo Secuenciotipo SPI SGI 540 Salmonella Typhimurium Monophasic variant Prophage Antibioticresistance Plasmids Genomic plasticity Mutations Flagellum Sequence SPI SGI Cuenca Arias, Paloma Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title | Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title_full | Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title_fullStr | Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title_full_unstemmed | Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title_short | Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica |
| title_sort | genomica comparativa de aislamientos clinicos colombianos de salmonella typhimurium variante monofasica |
| topic | Salmonella Typhimurium Variante monofásica Profago Antibióticoresistencia Plásmidos Plasticidad genómica Mutaciones Flagelo Secuenciotipo SPI SGI 540 Salmonella Typhimurium Monophasic variant Prophage Antibioticresistance Plasmids Genomic plasticity Mutations Flagellum Sequence SPI SGI |
| url | http://repositorio.uan.edu.co/handle/123456789/6383 |
| work_keys_str_mv | AT cuencaariaspaloma genomicacomparativadeaislamientosclinicoscolombianosdesalmonellatyphimuriumvariantemonofasica |
