Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)

The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) cro...

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Main Author:	Gómez Gómez, Alba Marcela
Other Authors:	Martínez Pachón, Diana
Format:	Trabajo de grado (Pregrado y/o Especialización)
Language:	spa
Published:	Universidad Antonio Nariño 2022
Subjects:	Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Enzymes
Online Access:	http://repositorio.uan.edu.co/handle/123456789/6376
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author	Gómez Gómez, Alba Marcela
author2	Martínez Pachón, Diana
author_facet	Martínez Pachón, Diana Gómez Gómez, Alba Marcela
author_sort	Gómez Gómez, Alba Marcela
collection	DSpace
description	The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) crop in minimal salt medium was determined. Genes and enzymes were detected in the genome of TN50 that may be associated with chlorpyrifos degradation and suggest that the bacterium may degrade the insecticide as a source of phosphorus and carbon. The chromatographic and liquid-liquid extraction technique for the monitoring of chlorpyrifos and its main degradation metabolite 3,5,6 trichloro-2-pyridinol was validated and the capacity of TN50 to use the insecticide as a sole carbon source was established. A 17.43% depletion of chlorpyrifos by P. aeruginosa was determined with glucose as an external carbon source and with prior metabolic activation in medium with glucose and chlorpyrifos, indicating that TN50 does not have the capacity to use the insecticide as a sole carbon source but can degrade the compound as an external nutrient source. Additional degradation studies could determine the potential of TN50 for bioremediation of this type of pollutants.
format	Trabajo de grado (Pregrado y/o Especialización)
id	repositorio.uan.edu.co-123456789-6376
institution	Repositorio Digital UAN
language	spa
publishDate	2022
publisher	Universidad Antonio Nariño
record_format	dspace
spelling	repositorio.uan.edu.co-123456789-63762024-10-09T22:47:06Z Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum) Gómez Gómez, Alba Marcela Martínez Pachón, Diana Vanegas Guerrero, Javier Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) crop in minimal salt medium was determined. Genes and enzymes were detected in the genome of TN50 that may be associated with chlorpyrifos degradation and suggest that the bacterium may degrade the insecticide as a source of phosphorus and carbon. The chromatographic and liquid-liquid extraction technique for the monitoring of chlorpyrifos and its main degradation metabolite 3,5,6 trichloro-2-pyridinol was validated and the capacity of TN50 to use the insecticide as a sole carbon source was established. A 17.43% depletion of chlorpyrifos by P. aeruginosa was determined with glucose as an external carbon source and with prior metabolic activation in medium with glucose and chlorpyrifos, indicating that TN50 does not have the capacity to use the insecticide as a sole carbon source but can degrade the compound as an external nutrient source. Additional degradation studies could determine the potential of TN50 for bioremediation of this type of pollutants. El insecticida clorpirifos se usa en variedad de cultivos agrícolas de todo el mundo y su aplicación a gran escala ha provocado contaminación ambiental. En este trabajo se determina la tasa de degradación de clorpirifos por la rizobacteria Pseudomonas aeruginosa (TN50) proveniente de cultivo de papa (Solanum tuberosum) en medio mínimo de sales. Se detectaron genes y enzimas en el genoma de TN50 que pueden estar asociados a la degradación de clorpirifos y sugieren que la bacteria podría degradar el insecticida como fuente de fósforo y carbono. Se validó la técnica cromatográfica y de extracción líquido-líquido para el seguimiento de clorpirifos y su principal metabolito de degradación 3,5,6-tricloro-2-piridinol y se estableció la capacidad de TN50 para usar el insecticida como única fuente de carbono. Se determinó la disminución del 17.43% de clorpirifos por P. aeruginosa con glucosa como fuente externa de carbono y con previa activación metabólica en medio con glucosa y clorpirifos, lo que indica que TN50 no tiene la capacidad de utilizar el insecticida como única fuente de carbono pero puede llegar a degradar el compuesto como fuente externa de nutrientes. Estudios adicionales de degradación podrían determinar el potencial de TN50 para la biorremediación de este tipo de contaminantes. Bioquímico(a) Pregrado Presencial Investigación 2022-04-30T00:09:27Z 2022-04-30T00:09:27Z 2021-11-24 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/6376 Ministerio de Agricultura y Desarrollo Rural, Minagricultura (2020). CADENA DE LA PAPA Indicadores e instrumentos. Unknown. https://sioc.minagricultura.gov.co/Papa/Documentos/2020-06-30%20Cifras%20Sectoria les.pdf. Consultado Marzo de 2021 Federación Colombiana de Productores de Papa, FEDEPAPA (2019). Informe de Gestión 2019. Unknown. https://fedepapa.com/wp-content/uploads/2021/09/BOLETIN-ECONOMICO-N%C2% B014.pdf. Consultado Noviembre de 2021 Shabbir, M., Singh, M., Maiti, S., Kumar, S., & Saha, S. K. (2018). Removal enactment of organo-phosphorous pesticide using bacteria isolated from domestic sewage. Bioresource Technology, 263, 280–288. https://doi.org/10.1016/j.biortech.2018.04.122 Aswathi, A., Pandey, A., & Sukumaran, R. K. (2019). Rapid degradation of the organophosphate pesticide – Chlorpyrifos by a novel strain of Pseudomonas nitroreducens AR-3. Bioresource Technology, 292(August), 122025. https://doi.org/10.1016/j.biortech.2019.122025 Cámara de Comercio de Bogotá (CCB) (2015). Manual papa. Vicepresidencia de Fortalecimiento Empresarial; Programa de Apoyo Agrícola y Agroindustrial. Unknown. http://hdl.handle.net/11520/14306. Consultado Marzo de 2020 Instituto Colombiano Agropecuario, ICA (2016). ESTADÍSTICAS DE COMERCIALIZACIÓN DE PLAGUICIDAS QUÍMICOS DE USO AGRÍCOLA 2016. Unknown. https://www.ica.gov.co/areas/agricola/servicios/regulacion-y-control-de-plaguicidas-qui micos/estadisticas/cartilla-plaguicidas-2016_22-01-18.aspx. Consultado Marzo de 2020 Pohanish, R. P. (2015). C. In Sittig’s Handbook of Pesticides and Agricultural Chemicals. 2, 91–195. https://doi.org/10.1016/B978-1-4557-3148-0.00003-0 Dow AgroSciences Colombia (2012). Lorsban, insecticida agrícola, concentrado emulsionante (EC). Etiqueta Web – Colombia.. Unknown. https://www.dowagro.com/content/dam/hdas/dowagro_colombia/pdfs/0901b80380988d 82.pdf. Consultado Marzo de 2020 Singh, B. K., Walker, A., Morgan, J. A. W., & Wright, D. J. (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology, 70(8), 4855–4863. https://doi.org/10.1128/AEM.70.8.4855-4863.2004 Li, X., Jiang, J., Gu, L., Ali, S. W., He, J., & Li, S. (2008). Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples. International Biodeterioration and Biodegradation, 62(4), 331–335. https://doi.org/10.1016/j.ibiod.2008.03.001 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar
spellingShingle	Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes Gómez Gómez, Alba Marcela Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_full	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_fullStr	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_full_unstemmed	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_short	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_sort	degradacion del insecticida clorpirifos por pseudomona aeruginosa rizobacteria aislada de cultivo de papa solanum tuberosum
topic	Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes
url	http://repositorio.uan.edu.co/handle/123456789/6376
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