Identificación por inmunofluorescencia de la expresión de proteínas estructurales del epitelio en células madre del folículo dental prueba piloto
The regeneration of the oral mucosa lost due to oral pathologies is a challenge, since that the affected oral tissues need to be restored to their original architecture. The cells of the dental follicle, are presented as an attractive option for the regeneration of tissues of the oral cavity. Previo...
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| Main Authors: | , |
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| Format: | Trabajo de grado (Pregrado y/o Especialización) |
| Language: | spa |
| Published: |
Universidad Antonio Nariño
2021
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| Subjects: | |
| Online Access: | http://repositorio.uan.edu.co/handle/123456789/2724 |
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| Summary: | The regeneration of the oral mucosa lost due to oral pathologies is a challenge, since
that the affected oral tissues need to be restored to their original architecture. The cells
of the dental follicle, are presented as an attractive option for the regeneration of
tissues of the oral cavity. Previously, the authors have used stem cells obtained
of oral tissues, investigating their regenerative potential. The purpose of the present study is
identify the expression of typical epithelial proteins Cytokeratin 8, Desmoglein, and
Desmoplaquin in stem cells obtained from dental follicle and in future studies its
potential application in the regeneration of oral mucosa.
METHODS
Previous consent, informed consent and institutional ethical endorsement, were obtained
dental germs of the third molar in the crown formation stage (n = 5), in patients
14 years old) the samples were stored and processed for the application of techniques
of cell culture, microscopic analysis evaluating cell viability, flow cytometry
to perform the cellular characterization and later the application of
immunohistochemistry, for subsequent identification of protein expression profiles
typical of the epithelium in dental follicle tissue.
RESULTS
The dental follicle stem cell cultures showed adherence to the surface of the
culture bottle, cell proliferation, allowing several passages to be made; this allowed
evaluate the primary culture obtained, which was characterized by the presentation of several
parameters: proliferation rate and expression of typical stromal cell markers
mesenchymal (MSC with adequate cell viability, observing in the results the
expression of the differentiation markers of the cells and the remarkable expression of the
Typical proteins of the epithelium in a greater proportion Desmoglein.
CONCLUSIONS
The preliminary results obtained in this pilot test provided evidence
immunohistochemistry of the expression of typical proteins of the epithelium Cytokeratin 8,
Desmoglein and Desmoplaquine, obtained from dental follicle tissue, a result that can be
relevant for tissue engineering research, constituting a useful complement
for use as a structural alternative for the generation of oral tissues or mucosa
oral. |
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