Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alv...
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Universidad Antonio Nariño
2021
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| _version_ | 1813940105007595520 |
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| author | Monzón Sánchez, Lina Mayerli |
| author2 | Alfonso Rodriguez, Camilo |
| author_facet | Alfonso Rodriguez, Camilo Monzón Sánchez, Lina Mayerli |
| author_sort | Monzón Sánchez, Lina Mayerli |
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| description | Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alveolar bone.
Cytokeratins 13 and 19. Result: The results found in this study demonstrated the heterogeneity and cell culture capacity of dental follicle cells. The immunofluorescence assay demonstrated high expression of cytokeratin 19 and moderate cytokeratin 13.
Conclusions: The found findings can explain from a basic point of view that this type of cells could differentiate to structurally similar epithelia such as oral mucosa and skin. In this study, the potential of dental follicle stem cells to differentiate into epithelial lineage was confirmed despite their mesenchymal nature and the expression of cytokeratins 13 and 19, being this the one with the highest expression. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-2723 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Antonio Nariño |
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| spelling | repositorio.uan.edu.co-123456789-27232024-10-21T12:35:03Z Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental Monzón Sánchez, Lina Mayerli Alfonso Rodriguez, Camilo Jaimes Monroy, Gustavo Inmunofluorescencia Citoqueratinas Foliculo dental Immunofluorescence Cytokeratins Dental follicle Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alveolar bone. Cytokeratins 13 and 19. Result: The results found in this study demonstrated the heterogeneity and cell culture capacity of dental follicle cells. The immunofluorescence assay demonstrated high expression of cytokeratin 19 and moderate cytokeratin 13. Conclusions: The found findings can explain from a basic point of view that this type of cells could differentiate to structurally similar epithelia such as oral mucosa and skin. In this study, the potential of dental follicle stem cells to differentiate into epithelial lineage was confirmed despite their mesenchymal nature and the expression of cytokeratins 13 and 19, being this the one with the highest expression. Introducción: El folículo dental es un tejido único compuesto por una condensación de células ectomesenquimales que rodea el germen dental en las primeras etapas de la formación del diente. Las células folículo dental pueden formar los 3 tipos de tejidos que constituyen el periodonto: ligamento periodontal, cemento y hueso alveolar. Citoqueratinas 13 y 19.Resultado: Los resultados encontrados en este estudio demostraron la heterogeneidad y la capacidad de cultivo celular de las células de folículo dental. El ensayo por inmunofluorescencia demostró alta expresión de citoqueratina 19 y moderada de citoqueratina 13. Conclusiones: Los hallazgos encontrados pueden explicar desde un punto de vista básico que este tipo de células podrían diferenciarse a epitelios estructuralmente similares como mucosa oral y piel. En este estudio se confirmó el potencial de las células madre del folículo dental , para diferenciarse a linaje epitelial a pesar de su naturaleza mesenquimal y la expresión de las citoqueratinas 13 y 19 siendo esta la de mayor expresión. Especialista en Periodoncia Especialización Presencial 2021-03-06T14:43:08Z 2021-03-06T14:43:08Z 2020-05-29 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/2723 1. Langer R. Advances in Tissue Engineering. EE.UU:; 2016. Report No.: PMS. 2. J. 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Cahill D., Marks, Jr. Tooth eruption: evidence for the central role of the dental follicle Journal of Oral Pathology 1980:9: 189-200 21. Wise GE, Lin F., Fan W. (1992).Culture and characterization of dental follicle cells from rat molars. Cell Tissue Res 267 :483-492 22. Kadkhoda Z., et al., Assessment of human periodontal ligament stem cell surface molecules and wisdom tooth follicle stem cell surface molecules Journal of Craniomaxillofacial Research 2017. 4(2):352-359. 23. Langer R., Vacanti JP Ingeniería de tejidos. Ciencia 1993; 260 (5110): 920 - 926. doi: 10.1126 / science.8493529 24. Shinsuke Ohba, Fumiko Yano, Ung-il Chung,Tissue engineering of bone and cartilage IBMS BoneKEy (2009), 405–419 25. Ratajczak J 1Las propiedades neurovasculares de las células madre dentales y su importancia en la ingeniería de tejidos dentales, Stem Cells Int. 2016; 2016: 9762871. doi: 10.1155 / 2016/9762871. 26. 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Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7. 31. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7. 32. Sharpe PT.Dental mesenchymal stem cells. Development. 2016 Jul 1;143(13):2273-80. 33. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7. 34. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C . Dental follicle stem cells and tissue engineering. J Oral Sci. 2010 Dec;52(4):541-52 35. Yao S, Pan F, Prpic V, Wise GE. Differentiation of Stem Cells in the Dental Follicle. Journal of dental research. 2008;87(8):767-771. 36. Navabazam AR, Sadeghian Nodoshan F, Sheikhha MH, Miresmaeili SM, Soleimani M, Fesahat F. Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament. Iran J Reprod Med. 2013 Mar;11(3):235-42. 37. Brizuela C. C.; Galleguillos, G. S.; Carrión, A. F.; Cabrera, P. C.; Luz, C. P. & Inostroza, S. C. Aislación y caracterización de células madre mesenquimales provenientes de pulpa y folículo dentario humano. Int. J. Morphol., 31(2):739-746, 2013. 38. Amila Brkic, Dental Follicle: Role In Development Of Odontogenic Cysts And Tumours, Journal Of Istanbul University Faculty Of Dentistry, Vol 48, No 1 (2014) 39. Meleti M, van der Waal I, Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connective tissue. The hypothesis of "focal ameloblastoma" Med Oral Patol Oral Cir Bucal 2013 18(1):e60-64 40. Honda MJ, Imaizumi M, Tsuchiya S, et al. Células madre del folículo dental e ingeniería de tejidos. J Oral Sci. 2010; 52: 541-52. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso a solo metadatos Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/closedAccess http://purl.org/coar/access_right/c_14cb application/pdf application/pdf Universidad Antonio Nariño Especialización en Periodoncia Facultad de Odontología Bogotá - Circunvalar |
| spellingShingle | Inmunofluorescencia Citoqueratinas Foliculo dental Immunofluorescence Cytokeratins Dental follicle Monzón Sánchez, Lina Mayerli Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title | Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title_full | Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title_fullStr | Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title_full_unstemmed | Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title_short | Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental |
| title_sort | identificacion por inmunofluorescencia de citoqueratinas 13 y 19 en celulas mensequimales del foliculo dental |
| topic | Inmunofluorescencia Citoqueratinas Foliculo dental Immunofluorescence Cytokeratins Dental follicle |
| url | http://repositorio.uan.edu.co/handle/123456789/2723 |
| work_keys_str_mv | AT monzonsanchezlinamayerli identificacionporinmunofluorescenciadecitoqueratinas13y19encelulasmensequimalesdelfoliculodental |
