Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos
Bacteriocins comprise a large number of peptides synthesized ribosomically by countless numbers of bacteria. Biosynthesis is carried out by structural genes that encode these peptides. The increasing bacterial resistance threatens human and animal health. Bacillus subtilis has been shown to produce...
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Universidad Antonio Nariño
2021
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| author | Landinez Velandia, Sandra Milena |
| author2 | López Pazos, Silvio Alejandro |
| author_facet | López Pazos, Silvio Alejandro Landinez Velandia, Sandra Milena |
| author_sort | Landinez Velandia, Sandra Milena |
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| description | Bacteriocins comprise a large number of peptides synthesized ribosomically by countless numbers of bacteria. Biosynthesis is carried out by structural genes that encode these peptides. The increasing bacterial resistance threatens human and animal health. Bacillus subtilis has been shown to produce a wide range of bacteriocins. The genus Pseudomonas sp. synthesizes different antimicrobial peptides to dominate over other competing organisms. P. extremeustralis species is a non-pathogenic Gram-negative bacterium, isolated from a pond in the Antarctic Peninsula. Twenty-one bacteriocin sequences were identified in silico in the genome of B. Subtilis ATCC 6633 and two associated sequences in the genome of P. extremeustralis CMPUJ U515. The physicochemical properties allowed establishing the molecular weight, the isoelectric point, the positively and negatively charged residues, the extinction coefficient, the stability index, the aliphatic index and the overall average of hydropaticity (GRAVY). The products obtained from PCR, resulted in three products that correspond to the bacteriocins Subtilin, Sublancin 168 and Subtilocin A. Finally, it was desired to establish the possible growth inhibitory effect of the protein extracts of B. subtilis ATCC 6633 and P. extremaustralis CMPUJ U515, for which a qualitative sensidisk test was performed on the strains of Escherichia coli ATCC 25922 and Staphylococcus aureus CMPUJ 080. Unfortunately no activity was found. In this investigation we concluded that Bacillus subtilis ATCC 6633 has biocontrol potential due to its bacteriocins which can be cloned by homologous recombination into yeast, to analyze its biological functionality. |
| format | Tesis y disertaciones (Maestría y/o Doctorado) |
| id | repositorio.uan.edu.co-123456789-1605 |
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| language | spa |
| publishDate | 2021 |
| publisher | Universidad Antonio Nariño |
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| spelling | repositorio.uan.edu.co-123456789-16052024-10-09T22:48:37Z Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos Landinez Velandia, Sandra Milena López Pazos, Silvio Alejandro Rojas Arias, Adriana Carolina péptidos codificados ribosomalmente, lantibióticos, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus. ribosomally coded peptides, lantibiotics, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus Bacteriocins comprise a large number of peptides synthesized ribosomically by countless numbers of bacteria. Biosynthesis is carried out by structural genes that encode these peptides. The increasing bacterial resistance threatens human and animal health. Bacillus subtilis has been shown to produce a wide range of bacteriocins. The genus Pseudomonas sp. synthesizes different antimicrobial peptides to dominate over other competing organisms. P. extremeustralis species is a non-pathogenic Gram-negative bacterium, isolated from a pond in the Antarctic Peninsula. Twenty-one bacteriocin sequences were identified in silico in the genome of B. Subtilis ATCC 6633 and two associated sequences in the genome of P. extremeustralis CMPUJ U515. The physicochemical properties allowed establishing the molecular weight, the isoelectric point, the positively and negatively charged residues, the extinction coefficient, the stability index, the aliphatic index and the overall average of hydropaticity (GRAVY). The products obtained from PCR, resulted in three products that correspond to the bacteriocins Subtilin, Sublancin 168 and Subtilocin A. Finally, it was desired to establish the possible growth inhibitory effect of the protein extracts of B. subtilis ATCC 6633 and P. extremaustralis CMPUJ U515, for which a qualitative sensidisk test was performed on the strains of Escherichia coli ATCC 25922 and Staphylococcus aureus CMPUJ 080. Unfortunately no activity was found. In this investigation we concluded that Bacillus subtilis ATCC 6633 has biocontrol potential due to its bacteriocins which can be cloned by homologous recombination into yeast, to analyze its biological functionality. Las bacteriocinas abarcan un gran número de péptidos sintetizados ribosómicamente por innumerable cantidad de bacterias. La biosíntesis es llevada a cabo por genes estructurales que codifican estos péptidos. La creciente resistencia bacteriana amenaza la salud humana y animal. Se ha demostrado que Bacillus subtilis produce amplia gama de bacteriocinas. El género Pseudomonas sp. sintetiza diferentes péptidos antimicrobianas para dominar sobre los otros organismos en competencia. La especie P. extremaustralis es una bacteria Gram negativa no patógena, aislada de un estanque de la Península Antártica. Se identificaron in silico 21 secuencias de bacteriocinas en el genoma de B. Subtilis ATCC 6633 y dos secuencias asociadas en el genoma de P. extremaustralis CMPUJ U515. Las propiedades fisicoquímicas permitieron establecer el peso molecular, el punto isoeléctrico, los residuos cargados positiva y negativamente, el coeficiente de extinción, el índice de estabilidad, el índice alifático y el promedio general de hidropaticidad (GRAVY). Los productos obtenidos de PCR, dio como resultado tres productos que corresponden a las bacteriocinas Subtilina, Sublancina 168 y Subtilocina A. Finalmente se deseaba establecer el posible efecto inhibidor de crecimiento de los extractos proteicos de B. subtilis ATCC 6633 y P. extremaustralis CMPUJ U515, para lo cual se realizó una prueba cualitativa con sensidiscos sobre las cepas de Escherichia coli ATCC 25922 y Staphylococcus aureus CMPUJ 080. Desafortunadamente no se encontró actividad. En esta investigación concluimos que Bacillus subtilis ATCC 6633 tiene potencial biocontrolador debido a sus bacteriocinas las cuales pueden clonarse mediante recombinación homologa en levadura, para analizar su funcionalidad biológica. Magister en Bioquímica Maestría Presencial 2021-02-22T15:28:23Z 2021-02-22T15:28:23Z 2020-05-29 Tesis y disertaciones (Maestría y/o Doctorado) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_bdcc http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/1605 Abriouel, H. F. (2011). Diversity and applications of Bacillus bacteriocins. 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| spellingShingle | péptidos codificados ribosomalmente, lantibióticos, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus. ribosomally coded peptides, lantibiotics, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus Landinez Velandia, Sandra Milena Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title | Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title_full | Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title_fullStr | Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title_full_unstemmed | Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title_short | Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos |
| title_sort | caracterizacion de bacteriocinas de bacillus subtilis atcc 6633 y pseudomonas extremaustralis cmpuj u515 en la perspectiva de control hacia patogenos humanos |
| topic | péptidos codificados ribosomalmente, lantibióticos, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus. ribosomally coded peptides, lantibiotics, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus |
| url | http://repositorio.uan.edu.co/handle/123456789/1605 |
| work_keys_str_mv | AT landinezvelandiasandramilena caracterizaciondebacteriocinasdebacillussubtilisatcc6633ypseudomonasextremaustraliscmpuju515enlaperspectivadecontrolhaciapatogenoshumanos |
