Agrobacterium-mediated transient expression for studying cassava resistance proteins

Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transien...

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Autores principales: Romero, Carlos, Fernández, Wendy, López, Camilo, Diaz, Paula
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Publicado: UNIVERSIDAD ANTONIO NARIÑO 2020
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Acceso en línea:https://revistas.uan.edu.co/index.php/saywa/article/view/690
https://repositorio.uan.edu.co/handle/123456789/11105
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author Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
author_facet Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
author_sort Romero, Carlos
collection DSpace
description Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen.
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publisher UNIVERSIDAD ANTONIO NARIÑO
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spelling repositorio.uan.edu.co-123456789-111052024-10-14T03:49:37Z Agrobacterium-mediated transient expression for studying cassava resistance proteins Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca Romero, Carlos Fernández, Wendy López, Camilo Diaz, Paula Bacteriosis vascular expresión transitoria genes de resistencia Inmunidad vegetal Xanthomonas axonopodis pv. Manihotis Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen. En este artículo se aborda el principio de la expresión transitoria mediada por Agrobacterium tumefaciens y su contribución en el estudio interacciones proteína-proteína en el campo de la fitopatología molecular. Adicionalmente, se expone el estado actual sobre la implementación de ensayos de expresión transitoria para el estudio de proteínas de resistencia de la yuca a la bacteriosis vascular. 2020-03-20 2024-10-10T02:55:01Z 2024-10-10T02:55:01Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Divulgación científica http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85 https://revistas.uan.edu.co/index.php/saywa/article/view/690 https://repositorio.uan.edu.co/handle/123456789/11105 spa https://revistas.uan.edu.co/index.php/saywa/article/view/690/591 https://creativecommons.org/licenses/by-nc-sa/4.0 http://purl.org/coar/access_right/c_abf2 application/pdf UNIVERSIDAD ANTONIO NARIÑO REVISTA SAYWA; Vol. 1 Núm. 2 (2019) 2744-8576 2711-1571
spellingShingle Bacteriosis vascular
expresión transitoria
genes de resistencia
Inmunidad vegetal
Xanthomonas axonopodis pv. Manihotis
Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
Agrobacterium-mediated transient expression for studying cassava resistance proteins
title Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_full Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_fullStr Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_full_unstemmed Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_short Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_sort agrobacterium mediated transient expression for studying cassava resistance proteins
topic Bacteriosis vascular
expresión transitoria
genes de resistencia
Inmunidad vegetal
Xanthomonas axonopodis pv. Manihotis
url https://revistas.uan.edu.co/index.php/saywa/article/view/690
https://repositorio.uan.edu.co/handle/123456789/11105
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AT fernandezwendy agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT lopezcamilo agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT diazpaula agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT romerocarlos expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT fernandezwendy expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT lopezcamilo expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT diazpaula expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
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