Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca

Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transien...

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Main Authors: Romero, Carlos, Fernández, Wendy, López, Camilo, Diaz, Paula
Format: Digital
Language:spa
Published: UNIVERSIDAD ANTONIO NARIÑO 2020
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Online Access:https://revistas.uan.edu.co/index.php/saywa/article/view/690
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author Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
author_facet Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
author_sort Romero, Carlos
collection OJS
description Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen.
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spelling revistas.uan.edu.co-article-6902021-08-18T03:36:54Z Agrobacterium-mediated transient expression for studying cassava resistance proteins Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca Romero, Carlos Fernández, Wendy López, Camilo Diaz, Paula Bacteriosis vascular expresión transitoria genes de resistencia Inmunidad vegetal Xanthomonas axonopodis pv. Manihotis Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen. En este artículo se aborda el principio de la expresión transitoria mediada por Agrobacterium tumefaciens y su contribución en el estudio interacciones proteína-proteína en el campo de la fitopatología molecular. Adicionalmente, se expone el estado actual sobre la implementación de ensayos de expresión transitoria para el estudio de proteínas de resistencia de la yuca a la bacteriosis vascular. UNIVERSIDAD ANTONIO NARIÑO 2020-03-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Divulgación científica application/pdf https://revistas.uan.edu.co/index.php/saywa/article/view/690 REVISTA SAYWA; Vol. 1 Núm. 2 (2019) 2744-8576 2711-1571 spa https://revistas.uan.edu.co/index.php/saywa/article/view/690/591 https://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle Bacteriosis vascular
expresión transitoria
genes de resistencia
Inmunidad vegetal
Xanthomonas axonopodis pv. Manihotis
Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula
Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title_alt Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_full Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title_fullStr Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title_full_unstemmed Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title_short Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title_sort expresion transitoria mediada por agrobacterium para el estudio de proteinas de resistencia de yuca
topic Bacteriosis vascular
expresión transitoria
genes de resistencia
Inmunidad vegetal
Xanthomonas axonopodis pv. Manihotis
topic_facet Bacteriosis vascular
expresión transitoria
genes de resistencia
Inmunidad vegetal
Xanthomonas axonopodis pv. Manihotis
url https://revistas.uan.edu.co/index.php/saywa/article/view/690
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AT fernandezwendy agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT lopezcamilo agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT diazpaula agrobacteriummediatedtransientexpressionforstudyingcassavaresistanceproteins
AT romerocarlos expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT fernandezwendy expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT lopezcamilo expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
AT diazpaula expresiontransitoriamediadaporagrobacteriumparaelestudiodeproteinasderesistenciadeyuca
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