Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans
Cocos nucifera has broad antimicrobial activity, recognized in herbal medicine as a medicine to treat diseases due to its medicinal properties. Streptococcus mutans is a facultative anaerobic microorganism, involved in carious processes. Objective: Determine the antibacterial effect of Cocos nucifer...
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Universidad Antonio Nariño
2023
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| author | Suárez Castro, Guadalupe Isabel Rey Ortiz, Liceth Valentina |
| author2 | Suárez Gélvez, Blanca Lynne |
| author_facet | Suárez Gélvez, Blanca Lynne Suárez Castro, Guadalupe Isabel Rey Ortiz, Liceth Valentina |
| author_sort | Suárez Castro, Guadalupe Isabel |
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| description | Cocos nucifera has broad antimicrobial activity, recognized in herbal medicine as a medicine to treat diseases due to its medicinal properties. Streptococcus mutans is a facultative anaerobic microorganism, involved in carious processes. Objective: Determine the antibacterial effect of Cocos nucifera essential oil against S. mutans. Materials and methods: In vitro experimental study. Essential oil was extracted by steam drag, using a Soxhlet system with distilled water as diluent, dilutions from 5% to 100% of the essential oil were made. Reference strain of S. mutans ATCC®25175 seeded on 5% blood agar was used. Sensitivity was evaluated with the agar diffusion method (Kirby-Bauer), with the formation of inhibition zones ≥18 mm. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and 50% lethal dose (LD50) were determined, based on the 0.5 turbidity pattern of the McFarland scale, equivalent to 1.5 x 108 UFC/ml. Results: They were obtained by evaluating the degree of sensitivity of S. mutans to concentrations between 5% and 100% of the essential oil of C. nucifera. Concentrations greater than 50% of the essential oil of C. nucifera generated inhibition zones ≥ 18 mm on S. mutans, a MBC of 145.6 mg/dl and an LD50 of 91.0 mg/dl were found. From a 30% dilution, inhibition zones ≥ 15 mm were observed. Conclusion: Cocos nucifera essential oil has an antibacterial effect on S. mutans. Keywords: Streptococcus mutans, Cocos nucifera, minimum inhibitory concentration, minimum bactericidal concentration, lethal dose 50. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-8866 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2023 |
| publisher | Universidad Antonio Nariño |
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| spelling | repositorio.uan.edu.co-123456789-88662024-10-09T22:39:52Z Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans Suárez Castro, Guadalupe Isabel Rey Ortiz, Liceth Valentina Suárez Gélvez, Blanca Lynne Vásquez Quijano, Adiel Alberto Streptococcus mutans Cocos nucifera Concentración mínima inhibitoria Concentración mínima bactericida Dosis letal 50 Streptococcus mutans Cocos nucifera Minimum inhibitory concentration Minimum bactericidal concentration Lethal dose 50 Cocos nucifera has broad antimicrobial activity, recognized in herbal medicine as a medicine to treat diseases due to its medicinal properties. Streptococcus mutans is a facultative anaerobic microorganism, involved in carious processes. Objective: Determine the antibacterial effect of Cocos nucifera essential oil against S. mutans. Materials and methods: In vitro experimental study. Essential oil was extracted by steam drag, using a Soxhlet system with distilled water as diluent, dilutions from 5% to 100% of the essential oil were made. Reference strain of S. mutans ATCC®25175 seeded on 5% blood agar was used. Sensitivity was evaluated with the agar diffusion method (Kirby-Bauer), with the formation of inhibition zones ≥18 mm. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and 50% lethal dose (LD50) were determined, based on the 0.5 turbidity pattern of the McFarland scale, equivalent to 1.5 x 108 UFC/ml. Results: They were obtained by evaluating the degree of sensitivity of S. mutans to concentrations between 5% and 100% of the essential oil of C. nucifera. Concentrations greater than 50% of the essential oil of C. nucifera generated inhibition zones ≥ 18 mm on S. mutans, a MBC of 145.6 mg/dl and an LD50 of 91.0 mg/dl were found. From a 30% dilution, inhibition zones ≥ 15 mm were observed. Conclusion: Cocos nucifera essential oil has an antibacterial effect on S. mutans. Keywords: Streptococcus mutans, Cocos nucifera, minimum inhibitory concentration, minimum bactericidal concentration, lethal dose 50. Cocos nucifera, posee amplia actividad antimicrobiana, reconocido en medicina herbaria como medicamento para tratar enfermedades por sus propiedades medicinales. Streptococcus mutans es un microorganismo anaerobio facultativo, involucrado en procesos cariosos. Objetivo: Determinar el efecto antibacteriano del aceite esencial de Cocos nucifera frente a S. mutans. Materiales y métodos: Estudio experimental in vitro. Se extrajo aceite esencial por arrastre de vapor, utilizando un sistema soxhlet con agua destilada como diluyente, se realizaron diluciones del 5% al 100% del aceite esencial. Se utilizó cepa de referencia de S. mutans ATCC®25175 sembrada en agar sangre 5%. La sensibilidad se evaluó con el método de difusión en agar (KirbyBauer), con formación de halos de inhibición ≥18 mm. Se determinó concentración mínima inhibitoria (CMI), concentración mínima bactericida (CMB) y dosis letal 50% (DL50), partiendo del patrón de turbidez 0.5 de la escala de McFarland, equivalente a 1.5 x 108 UFC/ml. Resultados: Se obtuvieron evaluando el grado de sensibilidad de S. mutans a concentraciones entre el 5% y el 100% del aceite esencial de C. nucifera. Concentraciones superiores al 50% del aceite esencial de C. nucifera generaron halos de inhibición ≥ 18 mm sobre S. mutans, se encontró una CMB de 145,6 mg/dl y una DL50 de 91,0 mg/dl. A partir de una dilución del 30% se observaron halos de inhibición ≥ 15 mm. Conclusión: El aceite esencial de Cocos nucifera tiene efecto antibacteriano sobre el S. mutans. Palabras clave: Streptococcus mutans, Cocos nucifera, concentración mínima inhibitoria, concentración mínima bactericida, dosis letal 50. Odontólogo(a) Pregrado Presencial Investigación 2023-11-28T21:35:35Z 2023-11-28T21:35:35Z 2023-11-10 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/8866 Ahmed, S., Mostafa, M., & El-Malt, M. (2020). Effect of Coconut Oil Pulling on Streptococcus Mutans Count in Saliva in Comparison with Chlorhexidine Mouthwash. Al-Azhar Dental Journal for Girls, 7(1), 7–11. Alban, G. P. G., Arguello, A. E. V., & Molina, N. E. C. (2020). Metodologías de investigación educativa (descriptivas, experimentales, participativas, y de investigaciónacción). Recimundo, 4(3), 163-173. Aqawi, M., Sionov, R. V., Gallily, R., Friedman, M., & Steinberg, D. (2021). Anti-bacterial properties of cannabigerol toward Streptococcus mutans. Frontiers in Microbiology, 12 (6), 1-15. Bohneberger, G., Machado, M. A., Debiasi, M. M., Dirschnabel, A. J., & Ramos, G. D. O. (2019). Fitoterápicos na odontologia, quando podemos utilizá-los? Brazilian Journal of Health Review, 2(4), 3504–351 Baqer, L. K. (2020). Antibacterial activity of sesame oil and coconut oil against the cariogenic Streptococcus mutans and Lactobacillus species-an in vitro study. Connect journals, 20 (1), 1961-1964. Beegum, P. S., Pandiselvam, R., Ramesh, S., Thube, S. H., Pandian, T. P., Khanashyam, A. C., Manikantan, M., & Hebbar, K. (2022). A critical appraisal on the antimicrobial, oral protective, and anti-diabetic functions of coconut and its derivatives. Quality Assurance and Safety of Crops & Foods, 14(2), 86–100. Bukhari, S., Jabeen, S., Ayub, N., Awan, U. L., Fatima, S., & Ishteyaque, S. (2019). Screening of cocos nucifera l. as a solution to antimicrobial resistance. International Journal of Biology and Biotechnology, 16(3), 775-782. Cerrato, M., Canales, D., Guevara, B., Chirinos, S., Ramirez, R. y Espinal, T. (2019, 18 julio). Frecuencia de caries dentales en pacientes de la clínica odontológica de la Universidad Tecnológica Centroamericana. Innovare: Revista de ciencia y tecnología, 8(1), 18-26. Chavez, S. G., Gómez, N. A., y Mestanza, M. (2022). Efecto del aceite esencial de Minthostachys mollis Kunth en la estabilidad oxidativa de aceite de sachainchi (Plukenetia hayllabambana). Revista chilena de nutrición, 49(2), 173-180. Casasola - Bado, M. La importancia de realizar una correcta tinción de Gram en la identificación bacteriana. (2022). Revista Colegio de Microbiólogos y Químicos Clínicos de Costa Rica, 27(2), 89–98. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf application/pdf Aremenia, Quindio , Colombia Universidad Antonio Nariño Odontología Facultad de Odontología Cúcuta |
| spellingShingle | Streptococcus mutans Cocos nucifera Concentración mínima inhibitoria Concentración mínima bactericida Dosis letal 50 Streptococcus mutans Cocos nucifera Minimum inhibitory concentration Minimum bactericidal concentration Lethal dose 50 Suárez Castro, Guadalupe Isabel Rey Ortiz, Liceth Valentina Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title | Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title_full | Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title_fullStr | Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title_full_unstemmed | Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title_short | Efecto antibacteriano del aceite esencial de Cocos nucifera frente a Streptococcus mutans |
| title_sort | efecto antibacteriano del aceite esencial de cocos nucifera frente a streptococcus mutans |
| topic | Streptococcus mutans Cocos nucifera Concentración mínima inhibitoria Concentración mínima bactericida Dosis letal 50 Streptococcus mutans Cocos nucifera Minimum inhibitory concentration Minimum bactericidal concentration Lethal dose 50 |
| url | http://repositorio.uan.edu.co/handle/123456789/8866 |
| work_keys_str_mv | AT suarezcastroguadalupeisabel efectoantibacterianodelaceiteesencialdecocosnuciferafrenteastreptococcusmutans AT reyortizlicethvalentina efectoantibacterianodelaceiteesencialdecocosnuciferafrenteastreptococcusmutans |
