Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.

The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged a...

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Main Author:	Duque Guevara, Stephany Valentina
Other Authors:	Reyes Guzmán, Edwin Alfredo
Format:	Trabajo de grado (Pregrado y/o Especialización)
Language:	spa
Published:	Universidad Antonio Nariño 2023
Subjects:	Bioquimica 574 38.23 D946e Biochemistry
Online Access:	http://repositorio.uan.edu.co/handle/123456789/8345
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author	Duque Guevara, Stephany Valentina
author2	Reyes Guzmán, Edwin Alfredo
author_facet	Reyes Guzmán, Edwin Alfredo Duque Guevara, Stephany Valentina
author_sort	Duque Guevara, Stephany Valentina
collection	DSpace
description	The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged as a promising strategy to selectively modulate NMDA receptor activity and counteract excitotoxicity. These peptides, from diverse sources such as snakes and spiders, act as specific ligands of the NMDA receptor and can function as agonists, antagonists or allosteric modulators, interacting with different binding sites on the receptor.
format	Trabajo de grado (Pregrado y/o Especialización)
id	repositorio.uan.edu.co-123456789-8345
institution	Repositorio Digital UAN
language	spa
publishDate	2023
publisher	Universidad Antonio Nariño
record_format	dspace
spelling	repositorio.uan.edu.co-123456789-83452024-10-09T22:37:16Z Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. Duque Guevara, Stephany Valentina Reyes Guzmán, Edwin Alfredo Cuervo Soto, Laura Inés Bioquimica 574 38.23 D946e Biochemistry The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged as a promising strategy to selectively modulate NMDA receptor activity and counteract excitotoxicity. These peptides, from diverse sources such as snakes and spiders, act as specific ligands of the NMDA receptor and can function as agonists, antagonists or allosteric modulators, interacting with different binding sites on the receptor. El receptor NMDA (N-metil-D-aspartato) desempeña un papel crucial en la función sináptica y la plasticidad neuronal, pero su hiperactivación puede llevar a la excitotoxicidad, un fenómeno implicado en diversas enfermedades neurodegenerativas. En este contexto, el uso de péptidos derivados de toxinas animales ha surgido como una estrategia prometedora para modular selectivamente la actividad del receptor NMDA y contrarrestar la excitotoxicidad. Bioquímico(a) Pregrado Presencial Investigación 2023-07-31T21:26:58Z 2023-07-31T21:26:58Z 2023-05-29 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/8345 Amico-Ruvio, S. A., Paganelli, M. A., Myers, J. M., & Popescu, G. K. (2012). Ifenprodil effects on GluN2B-containing glutamate receptors. Molecular Pharmacology, 82(6), 1074-1081. https://doi.org/10.1124/mol.112.078998. Asztély F, Gustafsson B. Ionotropic glutamate receptors: Their possible role in the expression of hippocampal synaptic plasticity. Mol Neurobiol. 1996;12(1):1–11. Aizenman E, Frosch MP, Lipton SA. Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat. J Physiol. 1988;396(1):75–91. Andreou LV. Isolation of plasmid DNA from bacteria. Methods Enzymol. 2013;529:135-42. doi: 10.1016/B978-0-12-418687-3.00010-0. PMID: 24011041. Albensi, B. C. (2007). The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Current pharmaceutical design, 13(31), 3185-3194. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (Eds.). (2005). Current Protocols in Molecular Biology. John Wiley & Sons. Baez, S. D. L. C., García del Barco, D., Hardy-Sosa, A., Guillen Nieto, G., Bringas-Vega, M. L., Llibre-Guerra, J. J., & Valdes-Sosa, P. (2021). Scalable Bio marker combinations for early stroke diagnosis: A systematic review. Frontiers in neurology, 12, 638693. Beaudoin, G. M., Lee, S. H., Singh, D., Yuan, Y., Ng, Y. G., Reichardt, L. F., & Arikkath, J. (2012). Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. Nature protocols, 7(9), 1741-1754. Beesley, S., Sullenberger, T., Pilli, J., Abbasi, S., Gunjan, A., & Kumar, S. S. (2019). Colocalization of distinct NMDA receptor subtypes at excitatory synapses in the entorhinal cortex. Journal of Neurophysiology, 121(1), 238-254. https://doi.org/10.1152/jn.00468.2018 Bermúdez Urrego, Y. S. (2022). Evaluation of the effect of the Pb53a a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK293 line. Bermudez, S and Reyes-Guzman EA. (2020). Tesis de pregrado. Evaluation of the effect of the Pb53a, a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK-293 line instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar
spellingShingle	Bioquimica 574 38.23 D946e Biochemistry Duque Guevara, Stephany Valentina Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title	Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title_full	Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title_fullStr	Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title_full_unstemmed	Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title_short	Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
title_sort	evaluacion de la expresion de subunidades recombinantes del receptor nmda en la linea celular hek 293
topic	Bioquimica 574 38.23 D946e Biochemistry
url	http://repositorio.uan.edu.co/handle/123456789/8345
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