Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium...
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Universidad Antonio Nariño
2023
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| author | Garcia Daza, Dayana Katerine |
| author2 | Losada Barragán, Mónica |
| author_facet | Losada Barragán, Mónica Garcia Daza, Dayana Katerine |
| author_sort | Garcia Daza, Dayana Katerine |
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| description | Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more
than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore
adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium-chain
free fatty acids, which has shown antiangiogenic, anti-inflammatory and proapoptotic properties in
various diseases. In this study, cell overload, oxidative stress, and the relative expression of PTEN,
CCND3, HIF 1α, and BCL2 tumor genes in T98G cells after octanoic and decanoic acid
supplementation for 3 days were evaluated. A reduction in cell turnover, superoxide levels and reactive oxygen species was found in both octanoic acid and decanoic acid treated cells. In addition, a reduction
of BCL2 in cells with decanoic acid and an increase in the expression of PTEN with low glucose
octanoic acid is evidenced, which suggests a recovery in the control of cell death. The study
demonstrates the potential in vitro use of medium chain fatty acids as adjuvant therapy |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-7386 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2023 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-73862024-10-09T23:07:16Z Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma Garcia Daza, Dayana Katerine Losada Barragán, Mónica Upegui Zapata, Yulieth Alexandra glioblastoma decanoic acid octanoic acid oxidative stress proliferation 572 glioblastoma decanoic acid octanoic acid oxidative stress proliferation Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium-chain free fatty acids, which has shown antiangiogenic, anti-inflammatory and proapoptotic properties in various diseases. In this study, cell overload, oxidative stress, and the relative expression of PTEN, CCND3, HIF 1α, and BCL2 tumor genes in T98G cells after octanoic and decanoic acid supplementation for 3 days were evaluated. A reduction in cell turnover, superoxide levels and reactive oxygen species was found in both octanoic acid and decanoic acid treated cells. In addition, a reduction of BCL2 in cells with decanoic acid and an increase in the expression of PTEN with low glucose octanoic acid is evidenced, which suggests a recovery in the control of cell death. The study demonstrates the potential in vitro use of medium chain fatty acids as adjuvant therapy El glioblastoma es el tumor más agresivo y maligno del cerebro, con una tasa de supervivencia no mayor a los 12 meses. En la actualidad se ha evidenciado resistencia a los tratamientos, por lo que hay una necesidad de explorar terapias adyuvantes de los ya existentes. Entre los que se encuentra la dieta cetogénica a base de ácidos grasos libres de cadena media que ha demostrado propiedades antiangiogénicas, antiinflamatorias y proapoptóticas en diversas enfermedades. En este estudio se evaluó la proliferación celular, el estrés oxidativo, y la expresión relativa de los genes tumorales PTEN, CCND3, HIF 1α y BCL2 en las células T98G después de la adición de ácido octanoico y decanoico durante 3 días. Se encontró una reducción en la proliferación celular, en los niveles de superóxido y especies reactivas de oxígeno tanto en células tratadas con ácido octanoico como con ácido decanoico. Además, se evidenció una reducción de BCL2 en células con ácido decanoico y un aumento en la expresión de PTEN con ácido octanoico bajo en glucosa lo que sugiere una recuperación en el control de muerte celular. El estudio demuestra el uso potencial in vitro de los ácidos grasos de cadena media como terapia adyuvante Bioquímico(a) Pregrado Presencial Investigación 2023-01-17T17:23:55Z 2023-01-17T17:23:55Z 2022-12-13 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/7386 abcam. (2021, December 29). ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476). Alves, A. L. v, Gomes, I. N. F., Carloni, A. C., Rosa, M. N., da Silva, L. S., Evangelista, A. F., Reis, R. M., & Silva, V. A. O. (2021). Role of glioblastoma stem cells in cancer therapeutic resistance: a perspective on antineoplastic agents from natural sources and chemical derivatives. Stem Cell Research & Therapy, 12(1), 206. https://doi.org/10.1186/s13287-021-02231-x Andersen, J., Westi, E., Jakobsen, E., Urruticoechea, N., Borges, K., & Aldana, B. (2021). Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Molecular Brain, 14(1), 132. Augustin, K., Khabbush, A., Williams, S., Eaton, S., Orford, M., Cross, J. H., Heales, S. J. R., Walker, M. C., & Williams, R. S. B. (2018). Mechanisms of action for the medium-chain triglyceride ketogenic diet in neurological and metabolic disorders. The Lancet Neurology, 17(1), 84–93. https://doi.org/10.1016/S1474-4422(17)30408-8 Avgerinos, K. I., Egan, J. M., Mattson, M. P., & Kapogiannis, D. (2020). Medium Chain Triglycerides induce mild ketosis and may improve cognition in Alzheimer’s disease. A systematic review and meta-analysis of human studies. Ageing Research Reviews, 58, 101001. https://doi.org/10.1016/j.arr.2019.101001 Brose, S. A., Marquardt, A. L., & Golovko, M. Y. (2014). Fatty acid biosynthesis from glutamate and glutamine is specifically induced in neuronal cells under hypoxia. Journal of Neurochemistry, 129(3), 400–412. https://doi.org/https://doi.org/10.1111/jnc.12617 Büschges, R., Weber, R. G., Actor, B., Lichter, P., Collins, V. P., & Reifenberger, G. (1999). Amplification and Expression of Cyclin D Genes (CCND1 CCND2 and CCND3) in Human Malignant Gliomas. Brain Pathology, 9(3), 435–442. https://doi.org/https://doi.org/10.1111/j.1750-3639.1999.tb00532.x Chang, H. T., Olson, L. K., & Schwartz, K. A. (2013). Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutrition & Metabolism, 10(1), 47. https://doi.org/10.1186/1743-7075-10-47 Chinot, O. L., Wick, W., Mason, W., Henriksson, R., Saran, F., Nishikawa, R., Carpentier, A. F., HoangXuan, K., Kavan, P., Cernea, D., Brandes, A. A., Hilton, M., Abrey, L., & Cloughesy, T. (2014). Bevacizumab plus Radiotherapy–Temozolomide for Newly Diagnosed Glioblastoma. New England Journal of Medicine, 370(8), 709–722. https://doi.org/10.1056/NEJMoa1308345 Cunnane, S. C., Trushina, E., Morland, C., Prigione, A., Casadesus, G., Andrews, Z. B., Beal, M. F., Bergersen, L. H., Brinton, R. D., de la Monte, S., Eckert, A., Harvey, J., Jeggo, R., Jhamandas, J. H., Kann, O., la Cour, C. M., Martin, W. F., Mithieux, G., Moreira, P. I., … Millan, M. J. (2020). Brain energy rescue: an emerging therapeutic concept for neurodegenerative disorders of ageing. Nature Reviews. Drug Discovery, 19(9), 609–633. https://doi.org/10.1038/s41573-020-0072-x instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar |
| spellingShingle | glioblastoma decanoic acid octanoic acid oxidative stress proliferation 572 glioblastoma decanoic acid octanoic acid oxidative stress proliferation Garcia Daza, Dayana Katerine Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title | Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title_full | Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title_fullStr | Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title_full_unstemmed | Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title_short | Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma |
| title_sort | evaluacion in vitro de los acidos octanoico y decanoico como tratamiento para el cancer de glioblastoma |
| topic | glioblastoma decanoic acid octanoic acid oxidative stress proliferation 572 glioblastoma decanoic acid octanoic acid oxidative stress proliferation |
| url | http://repositorio.uan.edu.co/handle/123456789/7386 |
| work_keys_str_mv | AT garciadazadayanakaterine evaluacioninvitrodelosacidosoctanoicoydecanoicocomotratamientoparaelcancerdeglioblastoma |
