Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)

The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the...

Full description

Saved in:

Bibliographic Details
Main Author:	Paez Almanza, Valentina
Other Authors:	Martinez Pachón, Diana
Format:	Trabajo de grado (Pregrado y/o Especialización)
Language:	spa
Published:	Universidad Antonio Nariño 2023
Subjects:	Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce.
Online Access:	http://repositorio.uan.edu.co/handle/123456789/7383
Tags:	Add Tag No Tags, Be the first to tag this record!

_version_	1812647219594002432
author	Paez Almanza, Valentina
author2	Martinez Pachón, Diana
author_facet	Martinez Pachón, Diana Paez Almanza, Valentina
author_sort	Paez Almanza, Valentina
collection	DSpace
description	The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the degradation of acetaminophen (ACE) by endophytic bacteria from lettuce from 3 municipalities of the Sabana de Bogotá (Cota, Mosquera and Funza). For this purpose, lettuce endophytes were selected in minimal salt medium containing acetaminophen, drug degradation was performed by HPLC, and bacteria were identified by 16s rRNA gene sequencing. The bacteria Klebsiella sp L1cp and Klebsiella pneumoniae L2cp use acetaminophen as a carbon and energy source for their growth. Strains L1cp and L2cp degraded 20% and 70% of acetamiphone at 50 mg/l in 60 h. Glucose addition affected ACE degradation, with L1cp degradation increased by 33% and L2cp degradation decreased by 53%. These results suggest the potential of lettuce endophytic bacteria to degrade acetaminophen and a possible mechanism of protection or detoxification of exogenous substances in plants.
format	Trabajo de grado (Pregrado y/o Especialización)
id	repositorio.uan.edu.co-123456789-7383
institution	Repositorio Digital UAN
language	spa
publishDate	2023
publisher	Universidad Antonio Nariño
record_format	dspace
spelling	repositorio.uan.edu.co-123456789-73832024-10-09T22:46:16Z Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) Paez Almanza, Valentina Martinez Pachón, Diana Vanegas Guerrero, Javier Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce. The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the degradation of acetaminophen (ACE) by endophytic bacteria from lettuce from 3 municipalities of the Sabana de Bogotá (Cota, Mosquera and Funza). For this purpose, lettuce endophytes were selected in minimal salt medium containing acetaminophen, drug degradation was performed by HPLC, and bacteria were identified by 16s rRNA gene sequencing. The bacteria Klebsiella sp L1cp and Klebsiella pneumoniae L2cp use acetaminophen as a carbon and energy source for their growth. Strains L1cp and L2cp degraded 20% and 70% of acetamiphone at 50 mg/l in 60 h. Glucose addition affected ACE degradation, with L1cp degradation increased by 33% and L2cp degradation decreased by 53%. These results suggest the potential of lettuce endophytic bacteria to degrade acetaminophen and a possible mechanism of protection or detoxification of exogenous substances in plants. La presencia y persistencia de productos farmacéuticos en la cadena alimentaria, especialmente en los cultivos alimentarios, puede tener efectos negativos sobre la salud humana y el medio ambiente. El acetaminofén en particular es un contaminante ambiental importante debido a su uso generalizado. El objetivo de este trabajo fue determinar la degradación del acetaminofén (ACE) por bacterias endófitas provenientes de lechuga de 3 municipios de la Sabana de Bogotá (Cota, Mosquera y Funza). Para este propósito, los endófitos de lechuga se seleccionaron en medio mínimo de sal que contenía acetaminofén, la degradación del fármaco se realizó por HPLC y las bacterias se identificaron por secuenciación del gen 16s rRNA. Las bacterias Klebsiella sp L1cp y Klebsiella pneumoniae L2cp utilizan acetaminofén como fuente de carbono y energía para su crecimiento. Las cepas L1cp y L2cp degradaron el 20 % y el 70 % del acetaminofen a 50 mg/l en 60 h. La adición de glucosa afectó la degradación de la ACE, ya que la degradación de L1cp aumentó en un 33 % y la de L2cp disminuyó en un 53 %. Estos resultados sugieren el potencial de las bacterias endófitas de la lechuga para degradar el acetaminofén y un posible mecanismo de protección o detoxificación de sustancias exógenas en las plantas. Bioquímico(a) Pregrado Presencial Investigación 2023-01-17T16:52:57Z 2023-01-17T16:52:57Z 2022-12-13 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/7383 Adegoke, A. A., Amoah, I. D., Stenström, T. A., Verbyla, M. E., & Mihelcic, J. R. (2018). Epidemiological evidence and health risks associated with agricultural reuse of partially treated and untreated wastewater: a review. Frontiers in public health, 6, 337. https://doi.org/10.3389/fpubh.2018.00337 Aissaoui, S., Ouled-Haddar, H., Sifour, M., Harrouche, K., & Sghaier, H. (2017). Metabolic and co-metabolic transformation of diclofenac by Enterobacter hormaechei D15 isolated from activated sludge. Current microbiology, 74(3), 381-388. https://doi.org/10.1007/s00284-016-1190-x Akay, C., & Tezel, U. (2020). Biotransformation of Acetaminophen by intact cells and crude enzymes of bacteria: A comparative study and modelling. Science of the total environment, 703, 134990. https://doi.org/10.1016/j.scitotenv.2019.134990 Akin-Ajani, O. D., Odeku, O. A., & Olumakinde-Oni, O. (2020). Evaluation of the mechanical and release properties of lactose and microcrystalline cellulose and their binary mixtures as directly compressible excipients in paracetamol tablets. Journal of Excipients and Food Chemicals, 11(2), 42-52. Ashraf, S., Afzal, M., Naveed, M., Shahid, M., & Ahmad Zahir, Z. (2018). Endophytic bacteria enhance remediation of tannery effluent in constructed wetlands vegetated with Leptochloa fusca. International Journal of Phytoremediation, 20(2), 121-128. DOI: 10.1080/15226514.2017.1337072. Badar, Z., Shanableh, A., El-Keblawy, A., Mosa, K. A., Semerjian, L., Mutery, A. A., ... & Semreen, M. H. (2022). Assessment of Uptake, Accumulation and Degradation of Paracetamol in Spinach (Spinacia oleracea L.) under Controlled Laboratory Conditions. Plants, 11(13), 1626. https://doi.org/10.3390/plants11131626 Bai, Y., Cui, Z., Su, R., & Qu, K. (2018). Influence of DOM components, salinity, pH, nitrate, and bicarbonate on the indirect photodegradation of acetaminophen in simulated coastal waters. Chemosphere, 205, 108-117. https://doi.org/10.1016/j.chemosphere.2018.04.087 Banco mundial (2017). Agua en la agricultura. https://www.bancomundial.org/es/topic/water-in-agriculture Bittsánszky, A., Gullner, G., Gyulai, G., & Komives, T. (2011). A case study: uptake and accumulation of persistent organic pollutants in Cucurbitaceae species. In Organic xenobiotics and plants (pp. 77-85). Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9852-8_4 Botero-Coy, A. M., Martínez-Pachón, D., Boix, C., Rincón, R. J., Castillo, N., Arias-Marín, L. P., ... & Hernandez, F. (2018). An investigation into the occurrence and removal of pharmaceuticals in Colombian wastewater. Science of the Total Environment, 642, 842-853. https://doi.org/10.1016/j.scitotenv.2018.06.088 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar
spellingShingle	Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce. Paez Almanza, Valentina Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title	Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title_full	Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title_fullStr	Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title_full_unstemmed	Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title_short	Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
title_sort	degradacion de acetaminofen en matrices acuosas por medio de bacterias endofitas provenientes del cultivo de lechuga lactuca sativa
topic	Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce.
url	http://repositorio.uan.edu.co/handle/123456789/7383
work_keys_str_mv	AT paezalmanzavalentina degradaciondeacetaminofenenmatricesacuosaspormediodebacteriasendofitasprovenientesdelcultivodelechugalactucasativa