Potencial de proliferación de las células madre de la papila apical en una biotinta a base de celulosa nanofibrilar

Objective: Evaluate the proliferation potential of aplical papilla stem cells in a nanofibrillar cellulose bioink. Materials and methods: In vitro study. The stem cells were obtained from a patient’s apical papilla, who provided express consent. The cell culture was completed up to fourth passage, t...

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Bibliographic Details
Main Authors:	Jiménez Romero, Karen Andrea, Muñoz, Andrés Horacio, Rivera Alfonso, Ayda, Tejedor Manjarrez, Héctor Fabio
Other Authors:	Alfonso Rodríguez, Camilo Andrés
Format:	Trabajo de grado (Pregrado y/o Especialización)
Language:	spa
Published:	Universidad Antonio Nariño 2022
Subjects:	Ingeniería Tisular Periodoncia Polifenoles Proliferación celular Viabilidad celular
Online Access:	http://repositorio.uan.edu.co/handle/123456789/7140
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Summary:	Objective: Evaluate the proliferation potential of aplical papilla stem cells in a nanofibrillar cellulose bioink. Materials and methods: In vitro study. The stem cells were obtained from a patient’s apical papilla, who provided express consent. The cell culture was completed up to fourth passage, they were mixed with nanofribillar cellulose from CELLINK Skin commercial enterprise and 4 groups with 3 1x1 constructs in a CELLINK Bio X Bioprinter. The days 1, 7 and 14 have been defined. Cellular characterization, Hematoxylin & eosin, PCNA and DAPI qualitative tests were conducted. Viability and cellular proliferation test were completed through Live-Dead and WSTI.