Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with pa...
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| Formato: | Trabajo de grado (Pregrado y/o Especialización) |
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Universidad Antonio Nariño
2022
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| Acceso en línea: | http://repositorio.uan.edu.co/handle/123456789/7108 |
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| _version_ | 1812647459183132672 |
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| author | Guevara Medina, Cesar Julian |
| author2 | Losada Barragán, Mónica |
| author_facet | Losada Barragán, Mónica Guevara Medina, Cesar Julian |
| author_sort | Guevara Medina, Cesar Julian |
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| description | Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with parasporins (PS) extracted from Bacillus thuringiensis (Bt) and different anticancer peptides (ACPs). In this work, the cy totoxic effect, and morphological changes present in HeLa cells by pure protein extracts of different Bt strains and synthetic peptides derived from PS6 called PS6DONE and PS6DTWO were evaluated. Additionally, cAMP levels in HeLa cells treated with the two peptides were quantified to determine their relationship with the adenylate cyclase (AC) signaling pathway by means of a direct competitive ELISA assay. The results show a high cytotoxic effect presented by protein extracts from parasporal inclusions agai nst the HeLa cell line associated with necrosis. On the other hand, a cytotoxic effect of the PS6DONE peptide and an increase in cAMP levels were observed, which were accompanied by morphological changes that suggested an autophagy process. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-7108 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-71082024-10-09T22:52:43Z Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa Guevara Medina, Cesar Julian Losada Barragán, Mónica Cáncer cérvix parasporinas HeLa Bacillus thuringiensis Cancer cervix parasporin HeLa Bacillus thuringiensis Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with parasporins (PS) extracted from Bacillus thuringiensis (Bt) and different anticancer peptides (ACPs). In this work, the cy totoxic effect, and morphological changes present in HeLa cells by pure protein extracts of different Bt strains and synthetic peptides derived from PS6 called PS6DONE and PS6DTWO were evaluated. Additionally, cAMP levels in HeLa cells treated with the two peptides were quantified to determine their relationship with the adenylate cyclase (AC) signaling pathway by means of a direct competitive ELISA assay. The results show a high cytotoxic effect presented by protein extracts from parasporal inclusions agai nst the HeLa cell line associated with necrosis. On the other hand, a cytotoxic effect of the PS6DONE peptide and an increase in cAMP levels were observed, which were accompanied by morphological changes that suggested an autophagy process. El cáncer de cuello uterino es una de las principales enfermedades en mujeres a nivel mundial, se han utilizado múltiples terapias no específicas e invasivas como la quimioterapia e histerectomía, que afectan células de linaje no cancerígeno. Por ello se han estudiado terapias alternativas específicas como el tratamiento con parasporinas (PS) extraídas de thuringiensis (Bt) y diferentes péptidos anticancerígenos (ACPs). En este trabajo, se Bacillus evalúa el efecto citotóxico y los cambios morfológicos presentes en células HeLa por extractos de inclusiones parasporales de diferentes cepas de Bt y péptidos sintéticos derivados de PS6 denominados PS6DONE y PS6DTWO. Adicionalmente, se cuantifican los ni veles de AMPc en células tratadas con el PS6DONE para determinar su relación con la vía de señalización del adenilato ciclasa (AC) por medio de un ELISA. Los resultados muestran un alto efecto citotóxico 3 presentado por los extractos de proteínas de inclusi asociados a necrosis ones parasporales frente a la línea celular HeLa . Por otra parte, se evidenció un efecto citotóxico del péptido PS6DONE y aumento de los niveles de AMPc, que fueron acompañados por cambios morfológicos que sugieren un proceso de autofa gia. Bioquímico(a) Pregrado Presencial Investigación 2022-10-12T16:58:02Z 2022-10-12T16:58:02Z 2022-07-08 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/7108 Appleby P, Beral V, Berrington de Gonzalez A, et al. Carcinoma of the cervix and tobacco smoking: collaborative reanalysis of individual data on 13,541 women with carcinoma of the cervix and 23,017 women without carcinoma of the cervix from 23 epidemiological studies. Int J Cancer. 2006;118(6):1481–1495 Bhatla, N., Aoki, D., Sharma, D. N., & Sankaranarayanan, R. (2018). Cancer of the cervix uteri. International Journal of Gynecology & Obstetrics, 143, 22–36. https://doi.org/10.1002/ijgo.12611 Bhatla, N., Berek, J. S., Cuello Fredes, M., Denny, L. A., Grenman, S., Karunaratne, K., Kehoe, S. T., Konishi, I., Olawaiye, A. B., Prat, J., & Sankaranarayanan, R. (2019). Revised FIGO staging for carcinoma of the cervix uteri. International Journal of Gynecology & Obstetrics, 145(1), 129–135. https://doi.org/10.1002/ijgo.12749 Brueggemann, L., Cribbs, L., Schwartz, J., Wang, M., Kouta, A., & Byron, K. (2018). Mechanisms of PKA-Dependent Potentiation of Kv7.5 Channel Activity in Human Airway Smooth Muscle Cells. International Journal of Molecular Sciences, 19(8), 2223. https://doi.org/10.3390/ijms19082223 Castella, C., Pauron, D., Hilliou, F., Trang, V. T., Zucchini-Pascal, N., Gallet, A., & Barbero, P. (2019). Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin, reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity. Biology Open. https://doi.org/10.1242/bio.037085 Crickmore, N.; Zeigler, D.R.; Schnepf, E.; Rie, J.; Lereclus, D.; Baum, J.; Bravo, A.; Dean, D.H. Bacillus thuringiensis Toxin Nomenclature. Available online: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ (Acceso en marzo de 2021). Deng, H., Hillpot, E., Mondal, S., Khurana, K. K., & Woodworth, C. D. (2018). HPV16Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-33865-2 Deng, H., Mondal, S., Sur, S., & Woodworth, C. D. (2019). Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. Journal of Cellular Physiology, 234(6), 76837694. https://doi.org/10.1002/jcp.28049 Elmore, S. (2007). Apoptosis: A Review of Programmed Cell Death. Toxicologic Pathology, 35(4), 495–516. https://doi.org/10.1080/01926230701320337 Epand, R. M., & Vogel, H. J. (1999). Diversity of antimicrobial peptides and their mechanisms of action. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1462(1–2), 1128. https://doi.org/10.1016/s0005-2736(99)00198-4 Felício, M. R., Silva, O. N., Gonçalves, S., Santos, N. C., & Franco, O. L. (2017). Peptides with Dual Antimicrobial and Anticancer Activities. Frontiers in Chemistry, 5. https://doi.org/10.3389/fchem.2017.00005 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar |
| spellingShingle | Cáncer cérvix parasporinas HeLa Bacillus thuringiensis Cancer cervix parasporin HeLa Bacillus thuringiensis Guevara Medina, Cesar Julian Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title | Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title_full | Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title_fullStr | Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title_full_unstemmed | Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title_short | Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa |
| title_sort | muerte celular inducida por parasporinas y peptidos sinteticos de la parasporina 6 de bacillus thuringiensis en celulas de cancer de cuello uterino y su relacion con la via de adenilato ciclasa |
| topic | Cáncer cérvix parasporinas HeLa Bacillus thuringiensis Cancer cervix parasporin HeLa Bacillus thuringiensis |
| url | http://repositorio.uan.edu.co/handle/123456789/7108 |
| work_keys_str_mv | AT guevaramedinacesarjulian muertecelularinducidaporparasporinasypeptidossinteticosdelaparasporina6debacillusthuringiensisencelulasdecancerdecuellouterinoysurelacionconlaviadeadenilatociclasa |
