“Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos”
Umbilical cord blood is used as a source of hematopoietic progenitor cells, for regeneration of the hemopoietic and immune systems in various hematological diseases; despite that, a low cellular dose limits its application in pediatric patients. In this st udy the standardization of cell expansion t...
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| Format: | Trabajo de grado (Pregrado y/o Especialización) |
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Universidad Antonio Nariño
2022
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| Online Access: | http://repositorio.uan.edu.co/handle/123456789/7080 |
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| author | Lara Rodriguez, Ana Milena |
| author2 | Arenas Suarez, Nelson Enrique |
| author_facet | Arenas Suarez, Nelson Enrique Lara Rodriguez, Ana Milena |
| author_sort | Lara Rodriguez, Ana Milena |
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| description | Umbilical cord blood is used as a source of hematopoietic progenitor cells, for regeneration of the hemopoietic and immune systems in various hematological diseases; despite that, a low cellular dose limits its application in pediatric patients. In this st udy the standardization of cell expansion technique of compensation for the dose infused cells is proposed, through ex vivo manipulation of hematopoietic progenitor cells from umbilical cord blood before transplantation. The expansion model was performed b y coculture with mesenchymal stem cells and bone marrow fetal tissues unexplored. The results show that the mesenchymal stem cells coculture studied allowed to maximize cell dose of hematopoietic progenitor cells between 5 and 16 folds, with high potenti al clonogenic. Moreover, evidence that treatment with gamma irradiation maintains the capacity to support hematopoietic mesenchymal stem cells from the three sources studied regarding the treatments without irradiation, promoting conditions in the ex vivo expansion system in coculture. It is expected that this standardized protocol will contribute to the development of ex vivo expansion larger scale, towards enabling its clinical use and expand further application in adults. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-7080 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-70802024-10-09T23:17:24Z “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” Lara Rodriguez, Ana Milena Arenas Suarez, Nelson Enrique Bonilla, Ximena Cordón umbilical Expansión ex vivo CD34+ Células madre mesenquimales Umb ilical cord Ex vivo expansión CD34 + Mesenchymal stem cells Umbilical cord blood is used as a source of hematopoietic progenitor cells, for regeneration of the hemopoietic and immune systems in various hematological diseases; despite that, a low cellular dose limits its application in pediatric patients. In this st udy the standardization of cell expansion technique of compensation for the dose infused cells is proposed, through ex vivo manipulation of hematopoietic progenitor cells from umbilical cord blood before transplantation. The expansion model was performed b y coculture with mesenchymal stem cells and bone marrow fetal tissues unexplored. The results show that the mesenchymal stem cells coculture studied allowed to maximize cell dose of hematopoietic progenitor cells between 5 and 16 folds, with high potenti al clonogenic. Moreover, evidence that treatment with gamma irradiation maintains the capacity to support hematopoietic mesenchymal stem cells from the three sources studied regarding the treatments without irradiation, promoting conditions in the ex vivo expansion system in coculture. It is expected that this standardized protocol will contribute to the development of ex vivo expansion larger scale, towards enabling its clinical use and expand further application in adults. La sangre de cordón umbilical es empleada como fuente de células progenitoras hematopoyéticas, para la regeneración de los sistemas hematopoyético e inmunológico en diversas enfermedades hematológicas; sin embargo, su baja dosis celular limita su uso a pacientes pediátricos. En este trabajo se propone la estandarización de una técnica de expansión celular para compensar la dosis de células infundidas, a través de la manipulación ex vivo de células progenitoras hematopoyéticas de sangre de cordón umbilical antes del trasplante. El modelo de expansión se realizó mediante un co-cultivo con células madre mesenquimales de médula ósea y de tejidos fetales poco explorados. Los resultados demuestran que el co-cultivo con las células madre mesenquimales estudiadas permite maximizar la dosis celular de células progenitoras hematopoyéticas entre 5 y 16 veces, con un alto potencial clonogénico. Además, se evidenció que el tratamiento con irradiación gamma, mantiene la capacidad de soporte hematopoyético de las células madre mesenquimales de las tres fuentes estudiadas respecto a los tratamientos sin irradiación, favoreciendo las condiciones en el sistema de expansión ex vivo en co-cultivo. Se espera que este protocolo estandarizado contribuya al desarrollo de la expansión ex vivo a mayor escala, posibilitando su uso clínico y ampliando su aplicación en adultos. Bioquímico(a) Pregrado Presencial Monografía 2022-10-11T22:45:10Z 2022-10-11T22:45:10Z 2022-07-06 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/7080 Andrzejewska, A., Lukomska, B., & Janowski, M. (2019). Concise Review: Mesenchymal Stem Cells: From Roots to Boost. Stem Cells (Dayton, Ohio), 37(7), 855–864. https://doi.org/10.1002/stem.3016 Belvedere, O., Feruglio, C., Malangone, W., Bonora, M. L., Donini, A., Dorotea, L., Tonutti, E., Rinaldi, C., Pittino, M., Baccarani, M., Del Frate, G., Biffoni, F., Sala, P., Hilbert, D. M., & Degrassi, A. (1999). Phenotypic Characterization of Immunomagnetically Purified Umbilical Cord Blood CD34+ Cells. Blood Cells, Molecules, and Diseases, 25(3), 141–146. https://doi.org/https://doi.org/10.1006/bcmd.1999.0239 Breems, D. A., Blokland, E. A. W., Siebel, K. E., Mayen, A. E. M., Engels, L. J. A., & Ploemacher, R. E. (1998). Stroma-Contact Prevents Loss of Hematopoietic Stem Cell Quality During Ex Vivo Expansion of CD34+ Mobilized Peripheral Blood Stem Cells. Blood, 91(1), 111–117. https://doi.org/https://doi.org/10.1182/blood.V91.1.111 Boulais, P. E., & Frenette, P. S. (2015). Making sense of hematopoietic stem cell niches. Blood, 125(17), 2621–2629. https://doi.org/10.1182/blood-2014-09-570192 Cancelas, J. A., Querol, S., Martín-Henao, G., Canals, C., Azqueta, C., Petriz, J., Inglés-Esteve, J., Amill, B., & Garcia, J. (1996). Isolation of hematopoietic progenitors. An approach to two different immunomagnetic methods at the lab scale. Pure and Applied Chemistry, 68(10), 1897–1901. https://doi.org/10.1351/pac199668101897 Chan, S. L., Choi, M., Wnendt, S., Kraus, M., Teng, E., Leong, H. F., & Merchav, S. (2007). Enhanced In Vivo Homing of Uncultured and Selectively Amplified Cord Blood CD34+ Cells by Cotransplantation with Cord Blood-Derived Unrestricted Somatic Stem Cells. Stem Cells, 25(2), 529–536. https://doi.org/10.1634/stemcells.2005-0639 Cordeiro Gomes, A., Hara, T., Lim, V. Y., Herndler-Brandstetter, D., Nevius, E., Sugiyama, T., Taniichi, S., Schlenner, S., Richie, E., Rodewald, H.-R., Flavell, R. A., Nagasawa, T., Ikuta, K., & Pereira, J. P. (2016). Hematopoietic Stem Cell Niches Produce Lineage-Instructive Signals to Control Multipotent Progenitor Differentiation. Immunity, 45(6), 1219–1231. https://doi.org/https://doi.org/10.1016/j.immuni.2016.11.004 Ding, L., Saunders, T. L., Enikolopov, G., & Morrison, S. J. (2012). Endothelial and perivascular cells maintain haematopoietic stem cells. Nature, 481(7382), 457–462. https://doi.org/10.1038/nature10783 Dominici, M., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F., Krause, D., Deans, R., Keating, A., Prockop, D., & Horwitz, E. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8(4), 315–317. https://doi.org/10.1080/14653240600855905 Emiloju, O. E., Potdar, R., Jorge, V., Gupta, S., & Varadi, G. (2019). Clinical Advancement and Challenges of ex vivo Expansion of Human Cord Blood Cells. Clinical Hematology International, 2(1), 18–26. https://doi.org/10.2991/chi.d.191121.001 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar |
| spellingShingle | Cordón umbilical Expansión ex vivo CD34+ Células madre mesenquimales Umb ilical cord Ex vivo expansión CD34 + Mesenchymal stem cells Lara Rodriguez, Ana Milena “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title | “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title_full | “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title_fullStr | “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title_full_unstemmed | “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title_short | “Estandarización de un protocolo de expansión ex vivo de células progenitoras hematopoyéticas aisladas de sangre de cordón umbilical en co-cultivo con células madre mesenquimales de diferentes tejidos” |
| title_sort | estandarizacion de un protocolo de expansion ex vivo de celulas progenitoras hematopoyeticas aisladas de sangre de cordon umbilical en co cultivo con celulas madre mesenquimales de diferentes tejidos |
| topic | Cordón umbilical Expansión ex vivo CD34+ Células madre mesenquimales Umb ilical cord Ex vivo expansión CD34 + Mesenchymal stem cells |
| url | http://repositorio.uan.edu.co/handle/123456789/7080 |
| work_keys_str_mv | AT lararodriguezanamilena estandarizaciondeunprotocolodeexpansionexvivodecelulasprogenitorashematopoyeticasaisladasdesangredecordonumbilicalencocultivoconcelulasmadremesenquimalesdediferentestejidos |
