Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with t...
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| Format: | Trabajo de grado (Pregrado y/o Especialización) |
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Universidad Antonio Nariño
2022
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| Online Access: | http://repositorio.uan.edu.co/handle/123456789/6809 |
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| author | Pacheco Martínez, Fabián Matías |
| author2 | Daza Bolaños, Carmen Alicia |
| author_facet | Daza Bolaños, Carmen Alicia Pacheco Martínez, Fabián Matías |
| author_sort | Pacheco Martínez, Fabián Matías |
| collection | DSpace |
| description | The study of the identification of biomarkers that are linked to the seminal quality of the
bull, are very important when quantifying proteins and sperm cells that determine the fertility of
the bovine male.
For this reason, a research internship was carried out in the Tecnho Sperm laboratory
with the aim of acquiring training in the techniques to evaluate the seminal quality of bovine
males from flow cytometry, western blot and real-time PCR, as conventional techniques. That
are carried out in laboratories whose purpose is to determine the biomarkers present in the
fertility of cattle.
Once the techniques were applied, it was observed that they are very effective and precise
to evaluate the viability of the semen from the different alterations that originate when the sperm
are subjected to thermal stress during the freezing process.
The hypothermic storage of bovine semen allows its cryopreservation without antibiotics,
but this process is limited by the high sensitivity of sperm at the time of freezing. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-6809 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-68092024-10-09T22:35:38Z Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación Pacheco Martínez, Fabián Matías Daza Bolaños, Carmen Alicia PCR en tiempo real citometría de flujo viabilidad células espermáticas real-time PCR Flow cytometry viability sperm cells The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with the aim of acquiring training in the techniques to evaluate the seminal quality of bovine males from flow cytometry, western blot and real-time PCR, as conventional techniques. That are carried out in laboratories whose purpose is to determine the biomarkers present in the fertility of cattle. Once the techniques were applied, it was observed that they are very effective and precise to evaluate the viability of the semen from the different alterations that originate when the sperm are subjected to thermal stress during the freezing process. The hypothermic storage of bovine semen allows its cryopreservation without antibiotics, but this process is limited by the high sensitivity of sperm at the time of freezing. El estudio de la identificación de los biomarcadores que estén vinculados con la calidad seminal del toro, son muy importantes al momento de cuantificar proteínas y células espermáticas que determinen la fertilidad del macho bovino. Por tal motivo se realizó una pasantía investigativa en el laboratorio Tecnho Sperm con el ánimo de adquirir un entrenamiento en las técnicas para evaluar la calidad seminal del macho bovino a partir de la citometría de flujo, western blot y PCR en tiempo real, como técnicas convencionales que se realizan en los laboratorios que tienen como fin determinar los biomarcadores presentes en la fertilidad del ganado bovino. Una vez aplicadas las técnicas se pudo observar que son muy eficaces y precisas para evaluar la viabilidad del semen a partir de las diferentes alteraciones que se originan al momento de someter los espermatozoides al estrés térmico durante el proceso congelación. El almacenamiento hipotérmico del semen del bovino permite su criopreservación sin antibióticos, pero este proceso está limitado a la alta sensibilidad que tiene los espermatozoides al momento de su congelación Médico(a) Veterinario(a) Pregrado Presencial Pasantía 2022-08-22T14:04:31Z 2022-08-22T14:04:31Z 2022-07-14 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/6809 Boe-Hansen, G. B., & Satake, N. (2019). An update on boar semen assessments by flow cytometry and CASA. Theriogenology, 137, 93–103. https://doi.org/10.1016/j.theriogenology.2019.05.043 Llavanera, M., Delgado-Bermúdez, A., Fernández-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., Barranco, I., & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of Animal Science and Biotechnology, 10(1), 1–11. https://doi.org/10.1186/s40104-019-0370-5 Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., Barranco, I., & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 1–14. https://doi.org/10.3390/antiox9080741 Meftahi, G. H. (2021). Applications of western blot technique : From bench to bedside. March, 1–9. https://doi.org/10.1002/bmb.21516 Memili, E., Moura, A. A., & Kaya, A. (2020). Metabolomes of sperm and seminal plasma associated with bull fertility. Animal Reproduction Science, March, 106355. https://doi.org/10.1016/j.anireprosci.2020.106355 Menezes, E. S. B., Badial, P. R., El Debaky, H., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., & Memili, E. (2020). Sperm miR15a and miR-29b are associated with bull fertility. Andrologia, 52(1), 1–11. https://doi.org/10.1111/and.13412 Purdy, P. H., Graham, J. K., & Azevedo, H. C. (2021). Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Animal Reproduction Science, 106846. https://doi.org/10.1016/j.anireprosci.2021.106846 Radtke, A., Dieckmann, K., Grobelny, F., Salzbrunn, A., & Oing, C. (2019). Expression of miRNA-371a-3p in seminal plasma and ejaculate is associated with sperm concentration. 469–474. https://doi.org/10.1111/andr.12664 Salas-Huetos, A., Blanco, J., Vidal, F., Godo, A., Grossmann, M., Pons, M. C., F-Fernández, S., Garrido, N., & Anton, E. (2015). Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile. Fertility and Sterility, 104(3), 591–601. https://doi.org/10.1016/j.fertnstert.2015.06.015 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Colombia(Popayán-cauca) Universidad Antonio Nariño Medicina Veterinaria Facultad de Medicina Veterinaria Popayán - Alto Cauca |
| spellingShingle | PCR en tiempo real citometría de flujo viabilidad células espermáticas real-time PCR Flow cytometry viability sperm cells Pacheco Martínez, Fabián Matías Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title | Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title_full | Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title_fullStr | Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title_full_unstemmed | Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title_short | Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación |
| title_sort | tecnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia informe de pasantia de investigacion |
| topic | PCR en tiempo real citometría de flujo viabilidad células espermáticas real-time PCR Flow cytometry viability sperm cells |
| url | http://repositorio.uan.edu.co/handle/123456789/6809 |
| work_keys_str_mv | AT pachecomartinezfabianmatias tecnicasmolecularesparadeterminarlacalidadseminaldelbovinosometidoacriotoleranciainformedepasantiadeinvestigacion |
