Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.

Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options i...

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Main Author:	Torres Viñuela, Valeria
Other Authors:	Losada Barragán, Mónica
Format:	Trabajo de grado (Pregrado y/o Especialización)
Language:	spa
Published:	Universidad Antonio Nariño 2022
Subjects:	Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Antimicrobial peptide Anticancer peptide Cervical cancer Cytotoxicity
Online Access:	http://repositorio.uan.edu.co/handle/123456789/6378
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author	Torres Viñuela, Valeria
author2	Losada Barragán, Mónica
author_facet	Losada Barragán, Mónica Torres Viñuela, Valeria
author_sort	Torres Viñuela, Valeria
collection	DSpace
description	Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options include anticancer peptides and proteins derived from antimicrobial peptides. The purpose of this study was to evaluate the cytotoxic effect and the cell death mechanism of the hypothetical protein PHP-1 of the psychrophilic microorganism Polaromonas sp. in the cervical cancer cell line. Through bioinformatic analysis, it was shown that PHP-1 exhibits structural characteristics typical of anti-cancer peptides and proteins. The cytotoxicity of PHP-1 was evaluated by an MTT test that showed a dose and time-dependent effect. The effects generated were additionally analyzed by means of an annexin V / PI labeling, indicating that the hypothetical protein induces apoptosis, which correlates with the observed morphological changes. The results of this work represent the first functional study of the hypothetical protein PHP-1 and suggest that PHP-1 is cytotoxic for the HeLa cell line by inducing cell apoptosis, highlighting its potential as an anticancer agent.
format	Trabajo de grado (Pregrado y/o Especialización)
id	repositorio.uan.edu.co-123456789-6378
institution	Repositorio Digital UAN
language	spa
publishDate	2022
publisher	Universidad Antonio Nariño
record_format	dspace
spelling	repositorio.uan.edu.co-123456789-63782024-10-09T22:56:49Z Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp. Torres Viñuela, Valeria Losada Barragán, Mónica Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options include anticancer peptides and proteins derived from antimicrobial peptides. The purpose of this study was to evaluate the cytotoxic effect and the cell death mechanism of the hypothetical protein PHP-1 of the psychrophilic microorganism Polaromonas sp. in the cervical cancer cell line. Through bioinformatic analysis, it was shown that PHP-1 exhibits structural characteristics typical of anti-cancer peptides and proteins. The cytotoxicity of PHP-1 was evaluated by an MTT test that showed a dose and time-dependent effect. The effects generated were additionally analyzed by means of an annexin V / PI labeling, indicating that the hypothetical protein induces apoptosis, which correlates with the observed morphological changes. The results of this work represent the first functional study of the hypothetical protein PHP-1 and suggest that PHP-1 is cytotoxic for the HeLa cell line by inducing cell apoptosis, highlighting its potential as an anticancer agent. El cáncer del cuello uterino presenta un alto índice de mortalidad entre las mujeres, siendo una de las neoplasias malignas con mayor incidencia. Las opciones terapéuticas actuales son altamente invasivas; por esto son necesarias alternativas de tratamiento con menores efectos secundarios e invasividad. Entre las nuevas posibles opciones terapéuticas se encuentran los péptidos y proteínas anticancerígenas derivadas de péptidos antimicrobianos. El propósito de este estudio fue evaluar el efecto citotóxico y el mecanismo de muerte celular de la proteína hipotética PHP-1 del microorganismo psicrófilo Polaromonas sp. en la línea celular de cáncer cérvico-uterino. Por medio de análisis bioinformáticos, se demostró que la PHP-1 exhibe características estructurales propias de los péptidos y proteínas anticancerígenas. La citotoxicidad de la PHP 1 fue evaluada mediante un ensayo MTT que mostró un efecto dosis y tiempo dependiente. Los efectos generados fueron complementariamente analizados por medio de una marcación de anexina V/PI, indicando que la proteína hipotética induce apoptosis, lo cual correlaciona con los cambios morfológicos observados. Los resultados de este trabajo representan el primer estudio funcional de la proteína hipotética PHP-1 y sugieren que la PHP-1 es citotóxica para la línea celular HeLa mediante la inducción de apoptosis celular, destacando su potencial como agente anticancerígeno. Bioquímico(a) Pregrado Presencial Investigación 2022-04-30T01:27:44Z 2022-04-30T01:27:44Z 2021-11-24 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/6378 Baindara, P., Korpole, S., & Grover, V. (2018). Bacteriocins: Perspective for the development of novel anticancer drugs. Applied Microbiology and Biotechnology, 102(24), 10393–10408. https://doi.org/10.1007/s00253-018-9420-8 Bravo, L. E. (2016). Estimating the incidence and mortality of cancer in Colombia: What are the best data for public policies? Colombia Medica, 71–73. https://doi.org/10.25100/cm.v47i2.2381 Brogden, K. A. (2005). Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology, 3(3), 238–250. https://doi.org/10.1038/nrmicro1098 Castro-Jiménez, M. Á., Vera-Cala, L. M., & Posso-Valencia, H. J. (2006). Epidemiología del cáncer de cuello uterino: Estado del arte. Revista Colombiana de Obstetricia y Ginecología, 57(3), 182–189. https://doi.org/10.18597/rcog.489 Chao, A., Yen, T.-C., & Lai, C.-H. (2014). Diagnosis and staging of cervical cancer. Advances in Cervical Cancer Management, 56–67. https://doi.org/10.2217/fmeb2013.13.71 Chen, Y., Jiang, S., Jin, Y., Yin, Y., Yu, G., Lan, X., Cui, M., Liang, Y., Wong, B. H. C., Guo, L., & Sun, H. (2012). Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom Agrocybe aegerita. Molecular Nutrition & Food Research, 56(11), 1729–1738. https://doi.org/10.1002/mnfr.201200316 Cibula, Pötter, Planchamp, Avall-Lundqvist, Fischerova, Haie-Meder, Köhler, Landoni, Lax, Lindegaard, Mahantshetty, Mathevet, McCluggage, McCormack, Naik, Nout, Pignata, Ponce, Querleu, … Raspollini. (2018). The european society of gynaecological oncology/european society for radiotherapy and oncology/european society of pathology guidelines for the management of patients with cervical cancer. Virchows Archiv, 472(6), 919–936. https://doi.org/10.1007/s00428-018-2362-9 Darcy, J. L., Lynch, R. C., King, A. J., Robeson, M. S., & Schmidt, S. K. (2011). Global distribution of polaromonas phylotypes - Evidence for a highly successful dispersal capacity. PLoS ONE, 6(8), e23742. https://doi.org/10.1371/journal.pone.0023742 de la Fuente-Núñez, C., Silva, O. N., Lu, T. K., & Franco, O. L. (2017). Antimicrobial peptides: Role in human disease and potential as immunotherapies. Pharmacology & Therapeutics, 178, 132–140. https://doi.org/10.1016/j.pharmthera.2017.04.002 Di Tommaso, P., Moretti, S., Xenarios, I., Orobitg, M., Montanyola, A., Chang, J.-M., Taly, J.-F., & Notredame, C. (2011). T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucleic Acids Research, 39(suppl), W13–W17. https://doi.org/10.1093/nar/gkr245 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar
spellingShingle	Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis Torres Viñuela, Valeria Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_full	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_fullStr	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_full_unstemmed	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_short	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_sort	evaluacion de la actividad anticancerigena de una proteina hipotetica recombinante php 1 de polaromonas sp
topic	Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis
url	http://repositorio.uan.edu.co/handle/123456789/6378
work_keys_str_mv	AT torresvinuelavaleria evaluaciondelaactividadanticancerigenadeunaproteinahipoteticarecombinantephp1depolaromonassp