Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral
Biomarkers, "are cellular, biochemical or molecular alterations that can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes or pharmacological responses to a therapeutic intervention. Objective: To determine t...
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Universidad Antonio Nariño
2021
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| author | Dussán Rivera, Henry Damián Silva Polonia, Dahian Lized Camacho Restrepo, Brenda |
| author2 | García Florez, Manuel |
| author_facet | García Florez, Manuel Dussán Rivera, Henry Damián Silva Polonia, Dahian Lized Camacho Restrepo, Brenda |
| author_sort | Dussán Rivera, Henry Damián |
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| description | Biomarkers, "are cellular, biochemical or molecular alterations that can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes or pharmacological responses to a therapeutic intervention. Objective: To determine the rates of cell proliferation in the well, moderately and p oorly differentiated stages of squamous cell cancer of the oral cavity. Materials and Methods: It corresponds to an experimental research, by the Immunofluorescence method, by means of image capture with the AxioCam I Cm1 coupled to the Axio imager Z2 Micr oscope with motorized z focus and obtained with the EC PlanNeofluar 40x/0.75 M27 objective. The Set 49 filter set was used, which detects the excitation at 358 nm and the emission at 463 nm. With the ZEN Digital Imaging program, the images were captured w ith 300 dpi resolution and in TIFF format. Results: The highest differentials in nuclei per parameter analyzed correspond to welldifferentiated (63% in N), poorly differentiated infiltrating (18.8% in S), moderately differentiated (71.9% in SR), poorly d ifferentiated infiltrating (7.3% in SI), well differentiated (10% in LR), moderately differentiated ulcerated and infiltrating (2.2% in LI) and well differentiated ulcerated and infiltrating (19% in I). Conclusions: 3 types of histological grade were deter mined in the sample; welldifferentiated, moderatelydifferentiated and poorly differentiated squamous cell carcinoma; identifying a greater number of nuclei in poorly differentiated squamous cell carcinoma. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-5035 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-50352024-10-09T23:03:10Z Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral Dussán Rivera, Henry Damián Silva Polonia, Dahian Lized Camacho Restrepo, Brenda García Florez, Manuel García Rojas, Claudia Lorena Lesión Bucal Carcinoma Escamocelular Ki67 Ciclo Celular Mouth Injury Cell Cycle Buccal Squamous Cell C arcinoma, Ki67 Cell Cycle Biomarkers, "are cellular, biochemical or molecular alterations that can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes or pharmacological responses to a therapeutic intervention. Objective: To determine the rates of cell proliferation in the well, moderately and p oorly differentiated stages of squamous cell cancer of the oral cavity. Materials and Methods: It corresponds to an experimental research, by the Immunofluorescence method, by means of image capture with the AxioCam I Cm1 coupled to the Axio imager Z2 Micr oscope with motorized z focus and obtained with the EC PlanNeofluar 40x/0.75 M27 objective. The Set 49 filter set was used, which detects the excitation at 358 nm and the emission at 463 nm. With the ZEN Digital Imaging program, the images were captured w ith 300 dpi resolution and in TIFF format. Results: The highest differentials in nuclei per parameter analyzed correspond to welldifferentiated (63% in N), poorly differentiated infiltrating (18.8% in S), moderately differentiated (71.9% in SR), poorly d ifferentiated infiltrating (7.3% in SI), well differentiated (10% in LR), moderately differentiated ulcerated and infiltrating (2.2% in LI) and well differentiated ulcerated and infiltrating (19% in I). Conclusions: 3 types of histological grade were deter mined in the sample; welldifferentiated, moderatelydifferentiated and poorly differentiated squamous cell carcinoma; identifying a greater number of nuclei in poorly differentiated squamous cell carcinoma. Los marcadores biológicos, “son alteraciones celulares, bioquímicas o moleculares que se pueden medir y evaluar objetivamente como un indicador de procesos biológicos normales, procesos patogénicos o respuestas farmacológicas a una intervención terapéutic a. Objetivo: Determinar los índices de proliferación celular en los estadios bien, moderadamente y pobremente diferenciado de cáncer escamocelular de la cavidad oral. Materiales y Métodos: Corresponde a una investigación de tipo experimental, por el métod Inmunofluorescencia, mediante captura de imágenes con la AxioCam I Cm1 acoplada al o de Microscopio Axio imager Z2 con enfoque en z motorizado y obtenidas con el objetivo EC Plan Neofluar 40x/0.75 M27. Se empleó el juego de filtros Set 49, que detecta la excitación de 358 nm y la emisión a 463 nm. Con el programa ZEN Digital Imaging se capturaron las imágenes con 300 dpi de resolución y en formato TIFF. Resultados: Los mayores diferenciales en nucleos por parámetro analizado corresponden a bien diferenci ado (63% en N), pobremente diferenciado infiltrante (18,8% en S), moderadamente diferenciado (71,9% en SR), pobremente diferenciado infiltrante (7,3% en SI), bien diferenciado (10% en LR), moderadamente diferenciado ulcerado e infiltrante (2,2% en li) y b en I) . Conclusiones: ien Diferenciado ulcerado e infiltrante (19% se determinaron 3 tipos de grado histológico en la muestra; el cáncer escamocelular bien diferenciado, moderadamente diferenciado y pobremente diferenciado; identificando una mayor diferenciado. cantidad de nucleos en el carcinoma escamocelular pobremente Odontólogo(a) Pregrado Presencial Investigación 2021-10-13T01:39:55Z 2021-10-13T01:39:55Z 2020-11-18 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/5035 Bernal D, L. A., & Hernández S, P. V. (2018). Detección temprana del cáncer oral en colombia e implicaciones para el sistema de salud. Bogotá: Unacional. Davila, L., Suarez, T., Pereira P, V., & Tomasi, R. (2019). BIOMARKERS BAX, BCL2, CD138, H3, KiIMMUNOEXPRESSION OF 67, MCM3 AND p53 IN ORAL LICHEN PLANUS. Montevideo (uruguay): Scielo.org. Filippi C, E. C., Oliveira, M. M., Jurkovski , B., & Callegari J, S. M. (2012). Nuclear Morphometric Analysis (NMA): Screening ofNuclear Morphometric Analysis (NMA): Screening of. Porto Alegre (Brasil): Plos one. García G, V., & Bascones M, A. (2009). Update in oral cancer. Madrid (españa): Scielo.org. Iamaroon, A., Khemaleelakul, U., Pongsiriwet, S., & Pintong, J. (2003). g. Co‐expression of p53 and Ki67 and lack of EBV expression in oral squamous cell carcinoma. (Tailandia): Journal Of oral Phatology & Medicine. IARC. (12 de mayo de 2019). i arc. Chiang Mai Recuperado el 12 de Septiembre de 2020, de iarc: https://gco.iarc.fr/today/data/factsheets/populations/170colombia- factsheets.pdf Jain, A., Maheshwari, V., Alam, K., Mehdi, G., & Sharma, S. (2009). factsheets.pdf Apoptosis in premalignant and malignant squamous ce ll lesions of the oral cavity: A light microscopic study. Aligarh (India): Indian Journal Phatology & Microbiology. Lobos N, J. F. (2019). Patologia d ela mucosa oral. Infermera virtual. Santiago de chile: Scielo.org. Obtenido de Infermera virtual. Megías, M., Molist, P., & Pombal, M. A. (2017). Atlas de Histología Animal y Vegetal. Celta de vigo (españa): U de vigo. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf application/pdf application/pdf application/pdf Huila (Colombia) Universidad Antonio Nariño Odontología Facultad de Odontología Neiva Buganviles |
| spellingShingle | Lesión Bucal Carcinoma Escamocelular Ki67 Ciclo Celular Mouth Injury Cell Cycle Buccal Squamous Cell C arcinoma, Ki67 Cell Cycle Dussán Rivera, Henry Damián Silva Polonia, Dahian Lized Camacho Restrepo, Brenda Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title | Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title_full | Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title_fullStr | Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title_full_unstemmed | Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title_short | Índice de Proliferación Celular con el marcador Ki67 En Cáncer Oral |
| title_sort | indice de proliferacion celular con el marcador ki67 en cancer oral |
| topic | Lesión Bucal Carcinoma Escamocelular Ki67 Ciclo Celular Mouth Injury Cell Cycle Buccal Squamous Cell C arcinoma, Ki67 Cell Cycle |
| url | http://repositorio.uan.edu.co/handle/123456789/5035 |
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