Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytoke...
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Universidad Antonio Nariño
2021
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| author | Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés |
| author2 | Alfonso Rodriguez, Camilo |
| author_facet | Alfonso Rodriguez, Camilo Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés |
| author_sort | Rodriguez Reyes, Diego Andrés |
| collection | DSpace |
| description | The stem cells of the apical papilla have excellent properties of
differentiation and great potential for use in tissue engineering protocols, without
However, the potential for epithelial differentiation has not been explored with this
cell type. The objective is to analyze the expression of cytokeratins 8, 13, and 19,
desmoplaquine and desmoglein by immunofluorescence. Materials and
Methods: Apical papilla tissue cell cultures were performed using
Explant technique of healthy donor patients. Characterization was performed
cell by flow cytometry, was analyzed by
immunofluorescence the expression of cytokeratins 8, 13 and 19, desmoplakine and
desmoglein. Results: The cells of the apical papilla expressed
cytokeratin 8, 13 and 19 desmoplaquine and desmoglein, observed through
the immunofluorescence technique, which was applied to the cells of the fourth pass
that demonstrated a cell viability of 86.76% by means of cytometry of
flow and trypan blue. Conclusions: The stem cells of the apical papilla
express epithelial markers and support the idea that they could be used as
an alternative source to develop artificial oral mucosa protocols. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-2725 |
| institution | Repositorio Digital UAN |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Antonio Nariño |
| record_format | dspace |
| spelling | repositorio.uan.edu.co-123456789-27252024-10-21T12:36:03Z Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés Alfonso Rodriguez, Camilo Jaimes Monroy, Gustavo citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytokeratins 8, 13, and 19, desmoplaquine and desmoglein by immunofluorescence. Materials and Methods: Apical papilla tissue cell cultures were performed using Explant technique of healthy donor patients. Characterization was performed cell by flow cytometry, was analyzed by immunofluorescence the expression of cytokeratins 8, 13 and 19, desmoplakine and desmoglein. Results: The cells of the apical papilla expressed cytokeratin 8, 13 and 19 desmoplaquine and desmoglein, observed through the immunofluorescence technique, which was applied to the cells of the fourth pass that demonstrated a cell viability of 86.76% by means of cytometry of flow and trypan blue. Conclusions: The stem cells of the apical papilla express epithelial markers and support the idea that they could be used as an alternative source to develop artificial oral mucosa protocols. Las células madre de la papila apical tienen excelentes propiedades de diferenciación y gran potencial de uso en protocolos de ingeniería tisular, sin embargo, el potencial de diferenciación epitelial no ha sido explorado con este tipo de células. El objetivo es analizar la expresión de citoqueratinas 8, 13, y 19, desmoplaquina y desmogleína mediante inmunofluorescencia. Materiales y Métodos: Se realizaron cultivos celulares de tejido de papila apical mediante técnica de explante de pacientes donadores sanos. Se realizó caracterización celular mediante citometría de flujo, se analizó por medio de inmunofluorescencia la expresión de citoqueratinas 8, 13 y 19, desmoplaquina y desmogleína. Resultados: Las células de la papila apical expresaron citoqueratina 8, 13 y 19 desmoplaquina y desmogleína, observado a través de la técnica inmunofluorescencia, la cual se le aplicó a las células del cuarto pase que demostraron una viabilidad celular del 86.76% por medio de citometría de flujo y azul de tripano. Conclusiones: Las células madre de la papila apical expresan marcadores epiteliales y soportan la idea que podrían ser usadas como una fuente alternativa para desarrollar protocolos de mucosa oral artificial. Odontólogo(a) Especialización Presencial 2021-03-06T14:54:20Z 2021-03-06T14:54:20Z 2020-05-29 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/2725 1- Kim R, Fasi A, Feinberg S. Soft tissue engineering in craniomaxillofacial surgery. Ann Maxillofac Surg. 2014; 4 (1): 4-8 2- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 3- Tonetti MS, Cortellini P, Pellegrini G1, Nieri M, Bonaccini D, Allegri M, et al. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. J Clin Periodontol. 2018; 45(1):78-88 4- Alfonso Rodriguez Camilo,Generación de Mucosa Oral Artificial Por Ingeniería Tisular,Granada España,Universidad de Granada,2014. 5- Karaöz E, Demircan PC, Sağlam O, Aksoy A, Kaymaz F, Duruksu G. Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells.Histochem Cell Biol. 2011; 136(4): 455-473. 6- Rao RS, Patil S, Ganavi BS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract. 2014; 15(1):127-36. 7- Holmstrup P, Plemons J, Meyle J.Non–plaque‐ induced gingival diseases.J Periodontol. 2018; 89(1):28-45 8- Sanchez-Quevedo M, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo PV, et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007; 22(6):631-40. 9- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 10- Garzon I, Alfonso-Rodríguez CA, Martínez-Gómez C, Carriel V, Martin-Piedra MA, Fernández-Valadés R, et al. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. Placenta. 2014; 35(12):994- 1000. 11- Garzón I, Miyake J, González-Andrades M, Carmona R, Carda C, SánchezQuevedo Mdel C, et al. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. Stem Cells Transl Med. 2013; 2(8):625-32 12- Martin-Piedra M, Garzon I, Oliveira A, Alfonso-Rodriguez CA, Carriel V, Scionti G, et al. Cell viability and proliferation capability of long-term human dental pulp stem cell cultures. Cytotherapy. 2014; 16(2):266-77 13- Martin-Piedra M, Garzon I, Oliveira AC, Alfonso-Rodríguez CA, SánchezQuevedo M, Campos A, et al. Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Cytotherapy. 2013; 15(4):507-18 14- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315-17. 15- Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71. 16- Bakopoulou A, About I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016; 2016:1- 20 17- Pang X, Zhuang Y, Li Z, Jing S, Cai Q, Zhang F, et al. Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways. Stem Cells Int. 2020; 2020:1-13. 18- Marañés C, Liceras E, Alaminos M, Fernández R, Ruiz A, Garzon I, et al. Generación de un sustituto de mucosa oral humana y comprobación de su viabilidad mediante ingeniería tisular. Cir Pediatr 2011; 24: 13-18. 19- Elias P, Matsuyoshi N, Wu H, Lin C, Wang Z, Brown B, et al. Desmoglein Isoform Distribution Affects Stratum Corneum Structure and Function. J Cell Biol. 2001; 153(2): 243-50. 20- Mahoney M, Sadowski S, Brennan D, Pikander P, Saukko P, Wahl J, et al. Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities. J Invest Dermatol. 2010; 130(4):968-78 21- Doğan A, Demirci S, Şahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell Biol Int. 2015; 39(1):94-103. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso a solo metadatos Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/closedAccess http://purl.org/coar/access_right/c_14cb application/pdf application/pdf application/pdf application/pdf Universidad Antonio Nariño Especialización en Periodoncia Facultad de Odontología Bogotá - Circunvalar |
| spellingShingle | citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title | Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title_full | Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title_fullStr | Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title_full_unstemmed | Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title_short | Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. |
| title_sort | identificacion por inmunofluorescencia de citoqueratinas 8 13 19 desmoplaquina y desmogleina en celulas madre de la papila apical ex vivo prueba piloto |
| topic | citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla |
| url | http://repositorio.uan.edu.co/handle/123456789/2725 |
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