Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytoke...
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| Main Authors: | , , |
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| Other Authors: | |
| Format: | Trabajo de grado (Pregrado y/o Especialización) |
| Language: | spa |
| Published: |
Universidad Antonio Nariño
2021
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| Subjects: | |
| Online Access: | http://repositorio.uan.edu.co/handle/123456789/2725 |
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| Summary: | The stem cells of the apical papilla have excellent properties of
differentiation and great potential for use in tissue engineering protocols, without
However, the potential for epithelial differentiation has not been explored with this
cell type. The objective is to analyze the expression of cytokeratins 8, 13, and 19,
desmoplaquine and desmoglein by immunofluorescence. Materials and
Methods: Apical papilla tissue cell cultures were performed using
Explant technique of healthy donor patients. Characterization was performed
cell by flow cytometry, was analyzed by
immunofluorescence the expression of cytokeratins 8, 13 and 19, desmoplakine and
desmoglein. Results: The cells of the apical papilla expressed
cytokeratin 8, 13 and 19 desmoplaquine and desmoglein, observed through
the immunofluorescence technique, which was applied to the cells of the fourth pass
that demonstrated a cell viability of 86.76% by means of cytometry of
flow and trypan blue. Conclusions: The stem cells of the apical papilla
express epithelial markers and support the idea that they could be used as
an alternative source to develop artificial oral mucosa protocols. |
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