Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulen...
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Universidad Antonio Nariño
2021
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| author | Santamaría Rodríguez, Brian David |
| author2 | Díaz Tatis, Paula Alejandra |
| author_facet | Díaz Tatis, Paula Alejandra Santamaría Rodríguez, Brian David |
| author_sort | Santamaría Rodríguez, Brian David |
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| description | Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulence factors. Some of these effectors, called TALEs (Transcription-Activator Like Effectors), enter the nucleus of host cells, bind specifically to host DNA sequences, called effector-binding elements (EBEs), and act as transcription factors to modulate host gene expression. The knowledge of the mode of action for TALEs in species as rice has allowed the development of biotechnological strategies to generate broad-spectrum resistance to pathogens. In this study, a trap promoter was developed that is activated by three predominant TALEs in Xpm strains that allow the expression of the resistance gene RXam2(MHV). Our results show that when TALEs 14, 20 and 22 are co-infiltrated with the synthetic promoter that contains the EBEs for this TALEs a hypersensitive response is produced in the infiltrated area in leaves of N. tabacum. These results represent an important achivement for the use of this strategy in future cassava breeding programs. |
| format | Trabajo de grado (Pregrado y/o Especialización) |
| id | repositorio.uan.edu.co-123456789-1600 |
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| publishDate | 2021 |
| publisher | Universidad Antonio Nariño |
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| spelling | repositorio.uan.edu.co-123456789-16002024-10-09T23:11:41Z Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis Santamaría Rodríguez, Brian David Díaz Tatis, Paula Alejandra Sanchez Ferro, Juan Sebastian bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible. cassava bacterial blight, trap promoter, hypersensitive response. Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulence factors. Some of these effectors, called TALEs (Transcription-Activator Like Effectors), enter the nucleus of host cells, bind specifically to host DNA sequences, called effector-binding elements (EBEs), and act as transcription factors to modulate host gene expression. The knowledge of the mode of action for TALEs in species as rice has allowed the development of biotechnological strategies to generate broad-spectrum resistance to pathogens. In this study, a trap promoter was developed that is activated by three predominant TALEs in Xpm strains that allow the expression of the resistance gene RXam2(MHV). Our results show that when TALEs 14, 20 and 22 are co-infiltrated with the synthetic promoter that contains the EBEs for this TALEs a hypersensitive response is produced in the infiltrated area in leaves of N. tabacum. These results represent an important achivement for the use of this strategy in future cassava breeding programs. Xanthomonas phaseoli pv. manihotis (Xpm) es el agente causal de la bacteriosis vascular de la yuca, enfermedad que puede generar pérdidas considerables en este cultivo. Esta bacteria posee proteínas efectoras que son inyectadas al interior de la célula hospedera a través del sistema de secreción tipo tres y que funcionan como factores de virulencia. Los efectores llamados TALEs (del inglés, Transcription-Activator Like Effector), ingresan al núcleo de las células hospederas, se unen de manera específica a las secuencias de ADN del hospedero, denominadas elementos de unión al efector (EBE) y actúan como factores de transcripción para modular la expresión de genes del hospedero. El conocimiento del modo de acción de los TALEs ha permitido desarrollar estrategias biotecnológicas para generar resistencia de amplio espectro a patógenos de plantas. En este estudio se elaboró un promotor trampa activado por tres TALEs predominantes en cepas de Xpm para inducir la expresión del gen de resistencia RXam2(MHV). Los resultados de los experimentos de expresión transitoria muestran que la co-infiltración de los TALEs 14, 20 y 22 junto con el promotor trampa que contiene los EBEs para estos tres efectores causan una respuesta hipersensible en el área infiltrada en hojas de N.tabacum. Bioquímico(a) Pregrado Presencial 2021-02-22T14:28:01Z 2021-02-22T14:28:01Z 2020-06-03 Trabajo de grado (Pregrado y/o Especialización) info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 http://repositorio.uan.edu.co/handle/123456789/1600 Aguilera, M. (2012). a Agroindustrial. Centro de Estudios Económicos Regionales (CEER) - Cartagena, 158. Retrieved from http://www.banrep.gov.co/docum/Lectura_finanzas/pdf/dtser_158.pdf Boch, J., Bonas, U., & Lahaye, T. (2014). 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Annual Review of Phytopathology, 55(1), 205–229. https://doi.org/10.1146/annurev-phyto-080516-035250 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ spa Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 application/pdf application/pdf Universidad Antonio Nariño Bioquímica Facultad de Ciencias Bogotá - Circunvalar |
| spellingShingle | bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible. cassava bacterial blight, trap promoter, hypersensitive response. Santamaría Rodríguez, Brian David Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title | Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title_full | Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title_fullStr | Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title_full_unstemmed | Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title_short | Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis |
| title_sort | induccion de la expresion del gen de resistencia de yuca rxam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv manihotis |
| topic | bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible. cassava bacterial blight, trap promoter, hypersensitive response. |
| url | http://repositorio.uan.edu.co/handle/123456789/1600 |
| work_keys_str_mv | AT santamariarodriguezbriandavid inducciondelaexpresiondelgenderesistenciadeyucarxam2medianteunpromotorinducibleportresefectorestipotaldexanthomonasphaseolipvmanihotis |
